中国蹄蝠中新甲型肠道病毒序列的鉴定与基因组特征。
Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China.
机构信息
Department of Microbiology, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
Qinghai Institute of Endemic Disease Prevention and Control, Xining, 811602, Qinghai, China.
出版信息
Virol J. 2022 May 15;19(1):80. doi: 10.1186/s12985-022-01806-1.
BACKGROUND
Bats were identified as a natural reservoir of emerging and re-emerging infectious pathogens threatening human health and life.
METHODS
This study collected 21 fecal samples of Hipposideros armiger in Mengla County of Xishuangbanna Prefecture Yunnan Province to combine one pool for viral metagenomic sequencing.
RESULTS
Two nearly complete genomes of parechoviruses, BPeV11 and BPeV20, were sequenced. Genome analysis revealed that BPeV11 and BPeV20 follow a 3-3-4 genome layout: 5' UTR-VP0-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3' UTR. The prevalence of BPev11 and BPev20 by Nested-PCR showed that 1 of 21 fecal samples was positive. Based on amino acid identity comparison and phylogenetic analysis of P1, 2C, and 3D, BPeV11 and BPeV20 were closely related to but distinct from FPeVs.
CONCLUSION
It was probably proposed to be a novel species in the genus Parechovirus of the family Picornaviridae. The isolation of BPev11 and BPev20 from H. armiger in China is the first complete genome of parechovirus isolations from bat feces of the genus Hipposideros.
背景
蝙蝠被认为是新兴和再现传染病原体的自然宿主，这些病原体威胁着人类的健康和生命。
方法
本研究收集了云南省西双版纳傣族自治州勐腊县 21 份蹄蝠粪便样本，混合为一个样本池进行病毒宏基因组测序。
结果
测序得到了两种近乎完整的副肠孤病毒基因组，BPeV11 和 BPeV20。基因组分析显示，BPeV11 和 BPeV20 遵循 3-3-4 基因组布局：5'UTR-VP0-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3'UTR。巢式 PCR 检测显示，21 份粪便样本中有 1 份为 BPeV11 和 BPev20 阳性。基于 P1、2C 和 3D 的氨基酸同一性比较和系统进化分析，BPeV11 和 BPeV20 与 FPeV 密切相关但又有所不同，可能被提议为副肠孤病毒属的一个新种。BPeV11 和 BPeV20 从中国蹄蝠中分离出来，是从蹄蝠属蝙蝠粪便中分离出的副肠孤病毒的第一个完整基因组。
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