Biomedical Research Institute, MEDIPOST Co. Ltd, 21, Daewangpangyo-ro 644-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 13494, Korea.
Tissue Eng Regen Med. 2020 Apr;17(2):193-202. doi: 10.1007/s13770-019-00237-4. Epub 2020 Feb 1.
Regeneration of soft tissue defects is essential for adipose tissue pathologies and disease, trauma, or injury-induced damage. Here, we show that umbilical cord blood-derived mesenchymal stem cells could potentially be tailored and used for the reconstruction of specific damaged sites. Adipogenesis can be exploited in soft tissue reconstruction. Also, primary cilia play a role in the control of adipogenesis.
The adipogenic differentiation capacity of mesenchymal stem cells (MSCs) was shown to influence ciliogenesis. MSCs transfected with intraflagellar transport 88 (IFT88) small interfering RNA (siRNA), which blocks the assembly and maintenance of cilia, were examined to confirm the relationship between adipogenesis and ciliogenesis. Also, 1,2-Bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), calcium chelator, inhibited the ciliogenesis of MSCs in adipogenic differentiation.
IFT88-knockdown led to decreased cilia formation and limitation of cilia elongation in adipogenesis. Additionally, intracellular calcium triggered cilia formation in MSCs adipogenesis. Interestingly, intracellular calcium cannot overcome the inhibition of adipogenesis caused by low numbers of cilia in MSCs.
Our data suggested that ciliogenesis was negatively regulated by Wnt5a/β-catenin signaling during adipogenesis. Thus, we suggest that calcium induction triggers adipogenesis and ciliogenesis.
软组织缺损的再生对于脂肪组织病理学、疾病、创伤或损伤引起的损伤至关重要。在这里，我们表明脐带血衍生的间充质干细胞可以被定制并用于特定受损部位的重建。脂肪生成可用于软组织重建。此外，初级纤毛在脂肪生成的控制中发挥作用。
证明间充质干细胞（MSCs）的脂肪生成分化能力会影响纤毛发生。转染了内鞭毛运输 88（IFT88）小干扰 RNA（siRNA）的 MSCs 被检查以确认脂肪生成和纤毛发生之间的关系，IFT88 siRNA 阻止纤毛的组装和维持。此外，1,2-双（2-氨基苯氧基）乙烷-N,N,N',N'-四乙酸四（乙酰氧甲基酯）（BAPTA-AM），钙螯合剂，抑制了 MSCs 在脂肪生成分化中的纤毛发生。
IFT88 敲低导致脂肪生成中纤毛形成减少和纤毛伸长受限。此外，细胞内钙在 MSCs 脂肪生成中触发纤毛形成。有趣的是，细胞内钙不能克服 MSCs 中纤毛数量少引起的脂肪生成抑制。
我们的数据表明，在脂肪生成过程中，纤毛发生受到 Wnt5a/β-catenin 信号的负调控。因此，我们建议钙诱导触发脂肪生成和纤毛发生。
