National Key Laboratory of Science and Technology on Tunable Laser, Harbin Institute of Technology, Harbin, 150080, China.
College of Art and Sciences, Northeast Agricultural University, Harbin, 150030, China.
Anal Sci. 2020 Aug 10;36(8):935-940. doi: 10.2116/analsci.19P476. Epub 2020 Jan 31.
This paper described how a high-yield, monodisperse Au nanobipyramids (Au NBs) sol was prepared by a seed-mediated method, and gold nanoparticles were assembled on the surface of a silicon wafer by self-assembly technology to obtain a solid SERS substrate. Scanning electron microscopy (SEM) showed that the average length of Au NBs was 34.31 nm, and the analysis enhancement factor (AEF) was approximately 7.3 × 10 with rhodamine 6G (R6G) used as a probe. SERS detection of chlortetracycline hydrochloride (CCH) in milk was performed utilizing the prepared Au NBs substrate, and the limit of detection was 0.01 mg/mL. In the range of 0.01 - 1 mg/mL, the mass concentration of CCH and the SERS signal intensity satisfied the linear relationship y = 258.467x + 150.501; the value of the correlation coefficient was 0.9785. In addition, the recovery of spiked samples fluctuated between 96.80 to 111.38%. These results proved that the method is simple and fast, and it is promising to be applied to the field detection of antibiotics in milk.
本文采用种子介导法制备了高产、单分散的金纳米棒（Au NBs）溶胶，并通过自组装技术将金纳米颗粒组装在硅片表面，得到了固体 SERS 基底。扫描电子显微镜（SEM）显示，Au NBs 的平均长度为 34.31nm，用罗丹明 6G（R6G）作为探针时，分析增强因子（AEF）约为 7.3×10。利用制备的 Au NBs 基底对牛奶中的盐酸金霉素（CCH）进行了 SERS 检测，检测限为 0.01mg/mL。在 0.01-1mg/mL 范围内，CCH 的质量浓度与 SERS 信号强度呈线性关系，y=258.467x+150.501，相关系数为 0.9785。此外，加标样品的回收率在 96.80%至 111.38%之间波动。这些结果表明，该方法简单快速，有望应用于牛奶中抗生素的现场检测。
