油对链脲佐菌素诱导的糖尿病大鼠心肌的保护作用
PROTECTIVE EFFECT OF OIL ON MYOCARDIUM IN STREPTOZOTOCIN-INDUCED DIABETIC RATS.
作者信息
Altun E, Avci E, Yildirim T, Yildirim S
机构信息
Balikesir University, School of Medicine - Dept. of Pathology, Balikesir, Turkey.
Balikesir University, School of Medicine - Dept. of Cardiology, Balikesir University, School of Medicine, Turkey.
出版信息
Acta Endocrinol (Buchar). 2019 Jul-Sep;15(3):289-294. doi: 10.4183/aeb.2019.289.
BACKGROUND
To evaluate the protective effect of oil (NSO) on the myocardium in streptozotocin-induced diabetic rats.
MATERIALS AND METHODS
Thirty-two 7-8-week-old female Wistar albino rats (300-350 g) were equally divided into 4 groups: nondiabetic untreated animals (control), diabetes mellitus (DM), NSO, and DM+NSO groups. For the induction of diabetes, 45 mg/kg streptozotocin was applied to the rats in the DM and DM+NSO groups as a single intraperitoneal dose. NSO (400 mg/kg) was orally administered through an intragastric catheter once a day over 21 days. Formalin-fixed, paraffin-embedded tissue sections of the myocardium were evaluated histopathologically and immunohistochemically.
RESULTS
Compared to the control, NSO, and DM+NSO groups, the myocardial tissue samples from the rats in the DM group had significantly higher myositis, hyaline degeneration, and Zenker's necrosis. Moreover, the Bcl-2 expressions were significantly higher in the control, NSO, and DM+NSO groups than in the DM group.
CONCLUSION
NSO has a protective effect on the myocardium of streptozotocin-induced diabetic rats, most likely via suppressing apoptosis.
背景
评估坚果籽油（NSO）对链脲佐菌素诱导的糖尿病大鼠心肌的保护作用。
材料与方法
将32只7 - 8周龄雌性Wistar白化大鼠（300 - 350克）平均分为4组：非糖尿病未治疗动物（对照组）、糖尿病（DM）组、NSO组和DM + NSO组。为诱导糖尿病，DM组和DM + NSO组大鼠腹腔注射一次45毫克/千克链脲佐菌素。NSO（400毫克/千克）通过胃内导管每天口服一次，持续21天。对福尔马林固定、石蜡包埋的心肌组织切片进行组织病理学和免疫组织化学评估。
结果
与对照组、NSO组和DM + NSO组相比，DM组大鼠的心肌组织样本出现明显更高程度的肌炎、透明变性和Zenker坏死。此外，对照组、NSO组和DM + NSO组的Bcl - 2表达明显高于DM组。
结论
NSO对链脲佐菌素诱导的糖尿病大鼠心肌具有保护作用，很可能是通过抑制细胞凋亡实现的。
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