Tang De-Rong, Li Cheng-Lin, Xu Ke-Ping, Wu Qing-Quan, Chen Qi-You, Lv Jun-Jie, Ji Jian, Zang Bao, Chen Chen, Gu Biao, Zhao Jian-Qiang
Department of Thoracic Surgery, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, China.
Front Oncol. 2020 Jan 17;9:1520. doi: 10.3389/fonc.2019.01520. eCollection 2019.
Pigment epithelium-derived factor (PEDF) is an oncogene found in various types of cancers. However, how PEDF affects the development of human esophageal squamous cell carcinoma (ESCC) is unknown. This study investigates the role of PEDF in ESCC cell proliferation, migration, and cell cycle both and . The PEDF expression was examined in patient tumor samples and ESCC cell lines. Short hairpin RNA technology was used to inhibit the PEDF expression in ESCC EC9706 and KYSE150 cells. cell proliferation and migration assays were performed. The effects of PEDF on tumor growth and progression were examined in murine subcutaneous xenograft tumor models. It was found that PEDF was overexpressed in esophageal cancer cells and patient tumor tissues compared to normal control samples. PEDF enhanced cell cycle progression and inhibited cell apoptosis. Knock down of PEDF inhibited esophageal cell proliferation and migration . Moreover, Inhibition of PEDF significantly reduced tumor growth and tumor size . These results indicate that PEDF induce tumorigenesis in ESCC and can be a potential therapeutic target for cancer treatment.
色素上皮衍生因子（PEDF）是在各类癌症中发现的一种癌基因。然而，PEDF如何影响人类食管鳞状细胞癌（ESCC）的发展尚不清楚。本研究探讨了PEDF在ESCC细胞增殖、迁移和细胞周期中的作用。检测了患者肿瘤样本和ESCC细胞系中PEDF的表达。利用短发夹RNA技术抑制ESCC EC9706和KYSE150细胞中PEDF的表达。进行了细胞增殖和迁移试验。在小鼠皮下异种移植肿瘤模型中检测了PEDF对肿瘤生长和进展的影响。结果发现，与正常对照样本相比，PEDF在食管癌细胞和患者肿瘤组织中过表达。PEDF促进细胞周期进程并抑制细胞凋亡。敲低PEDF可抑制食管细胞增殖和迁移。此外，抑制PEDF可显著降低肿瘤生长和肿瘤大小。这些结果表明，PEDF在ESCC中诱导肿瘤发生，可能成为癌症治疗的潜在靶点。
