Department of Dermatology, Medical University of Vienna, Vienna, Austria.
Department of Environmental Toxicology, University of California, Davis, CA, USA.
Exp Dermatol. 2020 Apr;29(4):376-379. doi: 10.1111/exd.14083. Epub 2020 Feb 18.
Long non-coding RNAs have been implicated in the regulation of a plethora of biological processes, yet it has been challenging to verify that they are truly not coding for proteins. Terminal differentiation-induced non-coding RNA (TINCR) is a 3.7-kilobase mRNA that is highly abundant in epidermal keratinocytes prior to cornification. Here, we report the presence of an evolutionarily conserved open reading frame in TINCR and the identification of peptides derived from this open reading frame in the proteome of human stratum corneum. Our results demonstrate that TINCR is a protein-coding RNA and suggest that the TINCR-encoded protein is involved in keratinocyte cornification.
长非编码 RNA 参与了大量生物过程的调节，但要验证它们是否真的不编码蛋白质，这一直具有挑战性。终端分化诱导的非编码 RNA（TINCR）是一种 3.7 千碱基的 mRNA，在角质形成细胞角化之前高度丰富。在这里，我们报告了 TINCR 中存在一个进化上保守的开放阅读框，并在人角质层蛋白质组中鉴定出源自该开放阅读框的肽。我们的结果表明 TINCR 是一种编码蛋白质的 RNA，并表明 TINCR 编码的蛋白质参与了角质形成细胞的角化。
