Opalski Adam S, Ruszczak Artur, Promovych Yurii, Horka Michał, Derzsi Ladislav, Garstecki Piotr
Institute of Physical Chemistry of the Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Micromachines (Basel). 2020 Jan 28;11(2):142. doi: 10.3390/mi11020142.
We demonstrate the utility of non-contact printing to fabricate the mAST-an easy-to-operate, microwell-based microfluidic device for combinatorial antibiotic susceptibility testing (AST) in a point-of-care format. The wells are prefilled with antibiotics in any desired concentration and combination by non-contact printing (spotting). For the execution of the AST, the only requirements are the mAST device, the sample, and the incubation chamber. Bacteria proliferation can be continuously monitored by using an absorbance reader. We investigate the profile of resistance of two reference strains, report the minimum inhibitory concentration (MIC) for single antibiotics, and assess drug-drug interactions in cocktails by using the Bliss independence model.
我们展示了非接触式打印在制造微阵列抗生素敏感性测试（mAST）方面的实用性，mAST是一种易于操作的、基于微孔的微流控装置，用于即时护理形式的组合抗生素敏感性测试（AST）。通过非接触式打印（点样），微孔中预先填充有任何所需浓度和组合的抗生素。对于AST的执行，唯一的要求是mAST装置、样品和孵育室。使用吸光度读数器可以连续监测细菌增殖情况。我们研究了两种参考菌株的耐药性概况，报告了单一抗生素的最低抑菌浓度（MIC），并使用布利斯独立模型评估了鸡尾酒中药物与药物之间的相互作用。
