Department of Food Science and Nutrition, Kyungpook National University, 1370 San-Kyuk Dong Puk-Ku, Daegu 41566, Korea.
Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 San-Kyuk Dong Puk-Ku, Daegu 41566, Korea.
Nutrients. 2020 Jan 29;12(2):352. doi: 10.3390/nu12020352.
Recently, there has been a global shift in diet towards an increased intake of energy-dense foods that are high in sugars. D-allulose has received attention as a sugar substitute and has been reported as one of the anti-obesity food components; however, its correlation with the intestinal microbial community is not yet completely understood. Thirty-six C57BL/6J mice were divided in to four dietary groups and fed a normal diet (ND), a high-fat diet (HFD, 20% fat, 1% cholesterol, w/w), and a HFD with 5% erythritol (ERY) and D-allulose (ALL) supplement for 16 weeks. A pair-feeding approach was used so that all groups receiving the high-fat diet would have the same calorie intake. As a result, body weight and body fat mass in the ALL group were significantly decreased toward the level of the normal group with a simultaneous decrease in plasma leptin and resistin. Fecal short-chain fatty acid (SCFA) production analysis revealed that ALL induced elevated total SCFA production compared to the other groups. Also, ALL supplement induced the change in the microbial community that could be responsible for improving the obesity based on 16S rRNA gene sequence analysis, and ALL significantly increased the energy expenditure in Day(6a.m to 6pm). Taken together, our findings suggest that 5% dietary ALL led to an improvement in HFD-induced obesity by altering the microbiome community.
最近，全球饮食结构发生了变化，人们摄入的高糖、高能量食物越来越多。D-阿洛酮糖作为一种代糖受到了关注，并且被报道为一种抗肥胖食品成分之一；然而，它与肠道微生物群落的关系尚不完全清楚。36 只 C57BL/6J 小鼠被分为四组饮食，分别喂食正常饮食（ND）、高脂肪饮食（HFD，20%脂肪，1%胆固醇，w/w）、HFD 加 5%赤藓糖醇（ERY）和 D-阿洛酮糖（ALL）补充剂 16 周。采用等热量喂养方法，使所有接受高脂肪饮食的组都具有相同的热量摄入。结果，ALL 组的体重和体脂肪质量显著下降，接近正常组水平，同时血浆瘦素和抵抗素也降低。粪便短链脂肪酸（SCFA）产生分析显示，与其他组相比，ALL 诱导了总 SCFA 产生的增加。此外，ALL 补充剂诱导了微生物群落的变化，这可能是基于 16S rRNA 基因序列分析改善肥胖的原因，并且 ALL 显著增加了白天（6am 到 6pm）的能量消耗。综上所述，我们的研究结果表明，5%的膳食 ALL 通过改变微生物群落，改善了 HFD 诱导的肥胖。
