Microbial Synthesis of Antibacterial Phenazine-1,6-dicarboxylic Acid and the Role of PhzG in GP72AN.

have been demonstrated to be environmentally friendly biocontrol strains, and most of them can produce phenazine compounds. Phenazine-1,6-dicarboxylic acid (PDC), with a potential antibacterial activity, is generally found in but not in . The present study aimed to explore the feasibility of PDC synthesis and the function of PhzG in . A PDC producer was constructed by replacing in with from . Through gene deletion, common start codon changing, gene silence, and in vitro assay, our result revealed that the yield of PDC in is associated with the relative expression of to and . In addition, it is found that PDC can be spontaneously synthesized without PhzG. This study provides an efficient way for PDC production and promotes a better understanding of PhzG function in PDC biosynthesis. Moreover, this study gives an alternative opportunity for developing new antibacterial biopesticides.