First Department of Otorhinolaryngology, Head and Neck Surgery, Hippocration Hospital, University of Athens, Athens, Greece.
Department of Otorhinolaryngology, Medical School, University of Patras, Patras, Greece.
Anticancer Res. 2020 Feb;40(2):807-811. doi: 10.21873/anticanres.14012.
BACKGROUND/AIM: Topoisomerase II alpha (TopoIIa) is a critical gene associated with response to chemo-therapeutic agents, such as anthracyclines, especially in breast adenocarcinoma. The aim of this study was to investigate the role of aberrant TopoIIa protein expression in laryngeal squamous cell carcinoma (LSCC).
Fifty (n=50) LSCC cases were enrolled in the study. Immunohistochemistry and a digital image analysis assay were implemented.
TopoIIa protein overexpression was observed in 32/50 (64%) cases, whereas low expression rates were detected in 18/50 (36%). TopoIIa overall expression presented strong association with the grade of the examined malignant tissues and borderline association with stage. TopoIIa overexpression correlated also with Human papillomavirus (HPV) positivity.
TopoIIa overexpression was observed in significant subsets of LSCCs, and correlated predominantly with the grade of differentiation. HPV persistent infection seems to be associated with increased TopoIIa protein expression. TopoIIa expression analysis appears to be critical in identifying sub-groups of patients eligible for specific chemotherapy.
背景/目的：拓扑异构酶 II alpha（TopoIIa）是与化疗药物反应相关的关键基因，特别是在乳腺腺癌中。本研究旨在探讨异常 TopoIIa 蛋白表达在喉鳞状细胞癌（LSCC）中的作用。
研究纳入了 50 例 LSCC 病例。采用免疫组织化学和数字图像分析检测方法。
32/50（64%）例 LSCC 中观察到 TopoIIa 蛋白过表达，而 18/50（36%）例 LSCC 中检测到低表达率。TopoIIa 的总体表达与所检查的恶性组织的分级呈强相关，与分期呈边缘相关。TopoIIa 过表达还与人乳头瘤病毒（HPV）阳性相关。
LSCC 中的显著亚组观察到 TopoIIa 过表达，且主要与分化程度相关。HPV 持续感染似乎与 TopoIIa 蛋白表达增加相关。TopoIIa 表达分析对于确定适合特定化疗的患者亚组非常重要。
