• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

拓扑异构酶 IIα（TopoIIa）：用于早期检测喉鳞状细胞癌的新的有前途的标志物：免疫组织化学研究。

Topoisomerase IIalpha (TopoIIa) A New Promising Marker for Early Detection of Laryngeal Squamous Cell Carcinoma: An Immunohistochemical Study.

机构信息

Department of Pathology, Faculty of Medicine, Cairo University,Cairo, Egypt.

出版信息

Asian Pac J Cancer Prev. 2023 Jun 1;24(6):2135-2139. doi: 10.31557/APJCP.2023.24.6.2135.

DOI:10.31557/APJCP.2023.24.6.2135
PMID:37378945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10505869/
Abstract

OBJECTIVE

the aim of this study is evaluation of Topo IIa expression in laryngeal squamous cell carcinomas and correlation of this expression with various clinicopathological parameters.

METHODS

ninety cases of laryngeal squamous cell carcinoma archived paraffin blocks were collected in the form of total laryngectomies. Each paraffin block was re-cut by rotatory microtome at 4 μm thickness and stained by hematoxylin and eosin for routine histopathological examination and on charged slides for immunohistochemistry using an automated staining system with antibodies against Topo IIa. Mainly nuclear and slightly cytoplasmic staining was considered positive. Percentage of positive Topo IIa cells was graded then grouped into low expression and overexpression.

RESULTS

Topo IIa overexpression was seen in 91.1% of cases, while low expression was noticed in the remaining 8.9% of cases. There were statistically significant correlations between Topo IIa expression and tumor histological grade, lymph node metastasis and T stage as well as statistically significant positive correlation between Topo IIa expression as we progress from normal to dysplastic/in situ up to malignant transformation.

CONCLUSION

High expression of Topo IIa may indicate more aggressive tumor and may play a role in tumorogenesis in laryngeal squamous cell carcinoma.

摘要

目的

本研究旨在评估喉鳞状细胞癌中 Topo IIa 的表达，并探讨其与各种临床病理参数的相关性。

方法

本研究收集了 90 例接受全喉切除术的喉鳞状细胞癌存档石蜡块。每个石蜡块均通过旋转式切片机以 4μm 的厚度进行重新切割，并通过苏木精和伊红染色进行常规组织病理学检查，以及通过免疫组织化学染色，使用自动染色系统对 Topo IIa 抗体进行 charged 载玻片染色。主要观察细胞核和轻微的细胞质染色为阳性。将阳性 Topo IIa 细胞的百分比进行分级，然后分为低表达和过表达。

结果

91.1%的病例中观察到 Topo IIa 过表达，而在其余 8.9%的病例中观察到低表达。Topo IIa 表达与肿瘤组织学分级、淋巴结转移和 T 分期之间存在统计学显著相关性，并且随着正常到异型增生/原位癌再到恶性转化的进展，Topo IIa 表达呈统计学显著正相关。

结论

Topo IIa 的高表达可能表明肿瘤更具侵袭性，并可能在喉鳞状细胞癌的肿瘤发生中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/f82e56b87235/APJCP-24-2135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/f7ffd5fd1c84/APJCP-24-2135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/09acda6d79ce/APJCP-24-2135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/793c9f21de78/APJCP-24-2135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/f82e56b87235/APJCP-24-2135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/f7ffd5fd1c84/APJCP-24-2135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/09acda6d79ce/APJCP-24-2135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/793c9f21de78/APJCP-24-2135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/996e/10505869/f82e56b87235/APJCP-24-2135-g004.jpg

相似文献

1
Topoisomerase IIalpha (TopoIIa) A New Promising Marker for Early Detection of Laryngeal Squamous Cell Carcinoma: An Immunohistochemical Study.拓扑异构酶 IIα（TopoIIa）：用于早期检测喉鳞状细胞癌的新的有前途的标志物：免疫组织化学研究。
Asian Pac J Cancer Prev. 2023 Jun 1;24(6):2135-2139. doi: 10.31557/APJCP.2023.24.6.2135.
2
Evaluation of topoisomerase IIa expression in pancreatic ductal adenocarcinoma: a pilot study using chromogenic in situ hybridization and immunohistochemistry on tissue microarrays.胰腺导管腺癌中拓扑异构酶IIa表达的评估：一项在组织微阵列上使用显色原位杂交和免疫组织化学的初步研究。
Pancreatology. 2007;7(1):45-52. doi: 10.1159/000101877. Epub 2007 Apr 18.
3
Topoisomerase II alpha expression and the Ki-67 labeling index correlate with prognostic factors in estrogen receptor-positive and human epidermal growth factor type-2-negative breast cancer.拓扑异构酶 II alpha 表达和 Ki-67 标记指数与雌激素受体阳性和人表皮生长因子受体 2 阴性乳腺癌的预后因素相关。
Breast Cancer. 2012 Oct;19(4):309-14. doi: 10.1007/s12282-011-0291-4. Epub 2011 Jul 2.
4
Prognostic importance of survivin, Ki-67, and topoisomerase IIα in ovarian carcinoma.生存素、Ki-67和拓扑异构酶IIα在卵巢癌中的预后重要性
Arch Gynecol Obstet. 2014 Feb;289(2):393-8. doi: 10.1007/s00404-013-3000-z. Epub 2013 Aug 22.
5
Expression of topoisomerase IIalpha, Ki-67, proliferating cell nuclear antigen, p53, and argyrophilic nucleolar organizer regions in vulvar squamous lesions.拓扑异构酶IIα、Ki-67、增殖细胞核抗原、p53及嗜银核仁组织区在女性外阴鳞状病变中的表达
Gynecol Oncol. 2002 Aug;86(2):192-9. doi: 10.1006/gyno.2002.6747.
6
Immunohistochemical study on topoisomerase IIalpha, Ki-67 and cytokeratin-19 in oral lichen planus lesions.口腔扁平苔藓病变中拓扑异构酶IIα、Ki-67和细胞角蛋白-19的免疫组织化学研究
Arch Dermatol Res. 2007 Jan;298(8):381-8. doi: 10.1007/s00403-006-0711-z. Epub 2006 Oct 28.
7
Immunohistochemical expression of topoisomerase II alpha and Her-2/neu in prostatic carcinoma and benign prostatic hyperplasia.拓扑异构酶IIα和Her-2/neu在前列腺癌和良性前列腺增生中的免疫组化表达
J Egypt Natl Canc Inst. 2008 Jun;20(2):158-67.
8
Topoisomerase II alpha expression in breast ductal invasive carcinomas and correlation with clinicopathological variables.
In Vivo. 2005 Sep-Oct;19(5):837-40.
9
DNA topoisomerase II-alpha immunoreactivity as a marker of tumor aggressiveness in invasive breast cancer.DNA拓扑异构酶II-α免疫反应性作为浸润性乳腺癌肿瘤侵袭性的标志物
Pathobiology. 2000 May-Jun;68(3):137-43. doi: 10.1159/000055914.
10
Topoisomerase IIalpha expression in breast cancer: correlation with outcome variables.拓扑异构酶IIα在乳腺癌中的表达：与预后变量的相关性。
Mod Pathol. 2000 May;13(5):542-7. doi: 10.1038/modpathol.3880094.

本文引用的文献

1
The Implication of Topoisomerase II Inhibitors in Synthetic Lethality for Cancer Therapy.拓扑异构酶II抑制剂在癌症治疗合成致死中的意义。
Pharmaceuticals (Basel). 2023 Jan 9;16(1):94. doi: 10.3390/ph16010094.
2
Marine-Derived Compounds Targeting Topoisomerase II in Cancer Cells: A Review.海洋来源的化合物靶向癌细胞拓扑异构酶 II：综述。
Mar Drugs. 2022 Oct 27;20(11):674. doi: 10.3390/md20110674.
3
Impact of Topoisomerases Complex Deregulation on Head and Neck Carcinoma Genomic Instability.拓扑异构酶复合物失调对头颈部癌基因组不稳定性的影响。
Anticancer Res. 2021 Jun;41(6):2773-2779. doi: 10.21873/anticanres.15058.
4
Potential Therapeutic Significance of Laminin in Head and Neck Squamous Carcinomas.层粘连蛋白在头颈部鳞状细胞癌中的潜在治疗意义
Cancers (Basel). 2021 Apr 15;13(8):1890. doi: 10.3390/cancers13081890.
5
Evaluation of Risk Factors for Laryngeal Squamous Cell Carcinoma: A Single-Center Retrospective Study.喉鳞状细胞癌危险因素评估：一项单中心回顾性研究
Front Oncol. 2021 Feb 25;11:606010. doi: 10.3389/fonc.2021.606010. eCollection 2021.
6
Topoisomerase IIa Protein Expression Patterns in Laryngeal Squamous Cell Carcinoma.喉鳞状细胞癌中拓扑异构酶 IIa 蛋白的表达模式。
Anticancer Res. 2020 Feb;40(2):807-811. doi: 10.21873/anticanres.14012.
7
Cancer statistics, 2020.癌症统计数据，2020 年。
CA Cancer J Clin. 2020 Jan;70(1):7-30. doi: 10.3322/caac.21590. Epub 2020 Jan 8.
8
Multiple simultaneous head and neck cancers in Lynch syndrome: Case report and literature review.林奇综合征患者同时发生多部位头颈部癌症：病例报告及文献综述
Laryngoscope. 2018 Dec;128(12):2759-2761. doi: 10.1002/lary.27259. Epub 2018 Oct 16.
9
The significance of Epstein Barr virus (EBV) & DNA topoisomerase II alpha (DNA-Topo II alpha) immunoreactivity in normal oral mucosa, oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC).EBV 与 DNA 拓扑异构酶 IIα 在正常口腔黏膜、口腔上皮异型增生和口腔鳞状细胞癌中的意义。
Diagn Pathol. 2008 Nov 20;3:45. doi: 10.1186/1746-1596-3-45.
10
Expression of human DNA Topoisomerase II-alpha in squamous cell carcinoma of the larynx and its correlation with clinicopathologic variables.人DNA拓扑异构酶II-α在喉鳞状细胞癌中的表达及其与临床病理变量的相关性。
Am J Clin Pathol. 2008 Dec;130(6):934-9. doi: 10.1309/AJCPROG61USKCBEI.