Uematsu T, Mizuno A, Suzuki Y, Sato R, Yamazaki T, Nakashima M
Department of Pharmacology, Hamamatsu University School of Medicine, Japan.
Ther Drug Monit. 1988;10(4):459-62. doi: 10.1097/00007691-198804000-00014.
A fluorescence polarization immunoassay (FPIA) procedure for measuring isepamicin, a new aminoglycoside antibiotic, was evaluated. The range of the assay studied was from 0.3-50 micrograms/ml and the calibration curve can be stored for at least 32 days. There was no interference from hemoglobin (up to 400 mg/dl) and bilirubin (up to 20 mg/dl). Within-run precision on each 20 replicates at 5, 15, and 25 micrograms/ml yielded a coefficient of variation (CV) of 1.44-2.52%; between-run precision on analyses at 5, 15, and 25 micrograms/ml over five periods yielded a CV of 1.26-1.59%. Cross-reactivities of the assay system with tobramycin and amikacin were null, but that with gentamicin, of which isepamicin is a derivative, was approximately 14-29%. Data obtained by microbiological assay and FPIA and by high-performance liquid chromatography and FPIA correlated significantly, with coefficients of correlation being 0.980 (n = 70) and 0.965 (n = 39), respectively. The FPIA offers a rapid, efficient, and accurate system for therapeutic monitoring of isepamicin plasma levels.
对一种用于测定新氨基糖苷类抗生素异帕米星的荧光偏振免疫分析法（FPIA）进行了评估。该分析方法的研究范围为0.3 - 50微克/毫升，校准曲线可保存至少32天。血红蛋白（高达400毫克/分升）和胆红素（高达20毫克/分升）无干扰。在5、15和25微克/毫升浓度下，每组20次重复测量的批内精密度变异系数（CV）为1.44 - 2.52%；在五个时间段内，对5、15和25微克/毫升浓度进行分析的批间精密度CV为1.26 - 1.59%。该分析系统与妥布霉素和阿米卡星的交叉反应性为零，但与异帕米星的衍生物庆大霉素的交叉反应性约为14 - 29%。通过微生物测定法与FPIA以及高效液相色谱法与FPIA获得的数据具有显著相关性，相关系数分别为0.980（n = 70）和0.965（n = 39）。FPIA为异帕米星血浆水平的治疗监测提供了一种快速、高效且准确的系统。
