Pesce M A, Bodourian S H
Ther Drug Monit. 1986;8(1):115-21. doi: 10.1097/00007691-198603000-00020.
A fluorescence polarization immunoassay (FPIA) procedure for measuring methotrexate was evaluated. The dynamic range of the assay is from 0.05 to 810 microM, and the calibration curve can be stored for at least 2 weeks. The FPIA procedure is automated and rapid; one result can be obtained in 18 min and five results in 25 min. There was no interference from hemoglobin (800 mg/dl), triglycerides (500 mg/dl), bilirubin (20 mg/dl), and protein (12.1 g/dl). Cross-reactivity with 7-hydroxy methotrexate and 2,4-diamino-N-methylpteroic acid was 0.6 and 44%, respectively. The coefficient of variation for the within-run and between-run precision was less than 5.0%. For the comparison studies, the samples were divided into four groups. The methotrexate concentrations in group 1 were 0.05-2.1 microM; in group 2, 2.2-9.3 microM; in group 3, 10-80 microM; and in group 4, greater than 80 microM. Linear regression analysis of the results obtained with the FPIA procedure and the enzyme multiplied immunoassay gave a correlation coefficient of at least 0.95 for all groups.
对一种用于测定甲氨蝶呤的荧光偏振免疫分析法（FPIA）进行了评估。该分析方法的动态范围为0.05至810微摩尔，校准曲线可保存至少2周。FPIA方法是自动化且快速的；18分钟可获得一个结果，25分钟可获得五个结果。血红蛋白（800毫克/分升）、甘油三酯（500毫克/分升）、胆红素（20毫克/分升）和蛋白质（12.1克/分升）均无干扰。与7 - 羟基甲氨蝶呤和2,4 - 二氨基 - N - 甲基蝶酸的交叉反应率分别为0.6%和44%。批内和批间精密度的变异系数均小于5.0%。在比较研究中，将样本分为四组。第1组中甲氨蝶呤浓度为0.05 - 2.1微摩尔；第2组为2.2 - 9.3微摩尔；第3组为10 - 80微摩尔；第4组大于80微摩尔。FPIA方法与酶放大免疫分析法所得结果的线性回归分析表明，所有组的相关系数至少为0.95。
