Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, Via S. Sofia 78, 95123, Catania, Italy.
Department of Economics, Unit of Statistical and Mathematical Sciences, University of Messina, Messina, Italy.
Sci Rep. 2020 Feb 3;10(1):1652. doi: 10.1038/s41598-020-58585-4.
Endothelin 1 (ET-1) has been shown to have a key role in homeostasis and function of endothelium and maybe fundamental in the relationship between coronary heart disease (CHD) and periodontitis. In this trial, we assessed the influence on serum and salivary ET-1 levels of gingival health, CHD, periodontitis, or a combination of periodontitis-CHD. Clinical and periodontal parameters, were collected from periodontitis patients (n = 34), CHD patients (n = 34), periodontitis + CHD patients (n = 34), and from healthy patients (n = 34) together with saliva and serum samples. The median concentrations of salivary and serum ET-1 were significantly higher in the CHD patients [serum: 1.4(1.1-1.6) pg/ml; saliva 1.2 (0.9-1.6) µmol/g, p < 0.01] and in the periodontitis + CHD patients [serum: 1.7 (1.2-21.8) pg/ml; salivary 1.4(1-1.6) µmol/g, p < 0.001] respect to periodontitis and control patients. Through a univariate regression analysis, c-reactive protein (CRP) and CHD (both p < 0.001) and periodontitis (p = 0.029) were statistically correlated with ET-1 in serum. The multivariate regression analysis demonstrated that only CRP was the statistically predictor of ET-1 in serum(p < 0.001). The multivariate regression analysis in saliva demonstrated that, regarding ET-1 levels the only predictor were CRP (p < 0.001) and total cholesterol (p = 0.042). The present study evidenced that subjects with CHD and periodontitis plus CHD had higher serum and salivary levels of ET-1 compared to subjects with periodontitis and healthy controls. Moreover, only CRP remained a major predictor of increased ET-1 concentrations in both serum and saliva.
内皮素 1（ET-1）在维持内皮细胞的稳态和功能方面具有关键作用，并且可能是冠心病（CHD）和牙周炎之间关系的基础。在这项试验中，我们评估了牙龈健康、CHD、牙周炎或牙周炎-CHD 联合对血清和唾液 ET-1 水平的影响。从牙周炎患者（n=34）、CHD 患者（n=34）、牙周炎+CHD 患者（n=34）和健康患者（n=34）中收集了临床和牙周参数，以及唾液和血清样本。CHD 患者的血清和唾液 ET-1 浓度中位数明显较高[血清：1.4（1.1-1.6）pg/ml；唾液 1.2（0.9-1.6）µmol/g，p<0.01]，牙周炎+CHD 患者的血清和唾液 ET-1 浓度中位数也明显较高[血清：1.7（1.2-21.8）pg/ml；唾液 1.4（1-1.6）µmol/g，p<0.001]。通过单变量回归分析，C 反应蛋白（CRP）和 CHD（均 p<0.001）和牙周炎（p=0.029）与血清中的 ET-1 呈统计学相关。多元回归分析表明，只有 CRP 是血清中 ET-1 的统计学预测因子（p<0.001）。唾液中的多元回归分析表明，关于 ET-1 水平，唯一的预测因子是 CRP（p<0.001）和总胆固醇（p=0.042）。本研究表明，与牙周炎和健康对照组相比，患有 CHD 和牙周炎加 CHD 的患者的血清和唾液中的 ET-1 水平更高。此外，只有 CRP 仍然是血清和唾液中 ET-1 浓度升高的主要预测因子。
