Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA.
Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, USA.
Nat Cell Biol. 2020 Feb;22(2):143-150. doi: 10.1038/s41556-019-0454-7. Epub 2020 Feb 3.
Deployment of RNA-guided DNA endonuclease CRISPR-Cas technology has led to radical advances in biology. As the functional diversity of CRISPR-Cas and parallel systems is further explored, RNA manipulation is emerging as a powerful mode of CRISPR-based engineering. In this Perspective, we chart progress in the RNA-targeting CRISPR-Cas (RCas) field and illustrate how continuing evolution in scientific discovery translates into applications for RNA biology and insights into mysteries, obstacles, and alternative technologies that lie ahead.
RNA 导向的 DNA 内切酶 CRISPR-Cas 技术的应用引领了生物学的重大进展。随着 CRISPR-Cas 和并行系统功能多样性的进一步探索，RNA 操作正在成为一种强大的基于 CRISPR 的工程模式。在本观点中，我们概述了 RNA 靶向 CRISPR-Cas（RCas）领域的进展，并说明了科学发现的持续进化如何转化为 RNA 生物学的应用，并深入了解未来的难题、障碍和替代技术。
