粘质沙雷氏菌调控突变体对正缬氨酸的积累
Norvaline accumulation by regulatory mutants of Serratia marcescens.
作者信息
Kisumi M, Sugiura M, Takagi T, Chibata I
出版信息
J Antibiot (Tokyo). 1977 Jan;30(1):111-7. doi: 10.7164/antibiotics.30.111.
PMID:320169
Abstract
A gene coding for desensitized L-threonine dehydratase was transduced with phage PS20 into a leucine accumulator of Serratia marcescens Sr41. The transductant converted L-threonine to alpha-ketobutyrate, a precursor of both norvaline and isoleucine. An isoleucine-valine auxotroph of the transductant accumulated large amount of norvaline from L-threonine as well as from D-threonine.
摘要
用噬菌体PS20将编码脱敏L-苏氨酸脱水酶的基因转导到粘质沙雷氏菌Sr41的亮氨酸积累突变体中。转导子将L-苏氨酸转化为α-酮丁酸，它是正缬氨酸和异亮氨酸的前体。转导子的异亮氨酸-缬氨酸营养缺陷型从L-苏氨酸以及D-苏氨酸中积累了大量正缬氨酸。
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