Department of Urology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):639-646. doi: 10.26355/eurrev_202001_20040.
MicroRNAs (miRNAs) are endogenous, non-coding RNAs, which exert crucial functions in regulating biological progressions. Previous studies have demonstrated the anti-tumor effect of miRNA-215-5p. However, its specific role in influencing the progression of prostate cancer (PCa) remains unclear. This study aims to uncover the regulatory effect of miRNA-215-5p on the metastasis and prognosis of PCa.
MiRNA-215-5p levels in collected PCa tissues (n=52) and paracancerous tissues (n=52) were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relationship between miRNA-215-5p level and pathological indexes, as well as overall survival of PCa patients, was analyzed. Regulatory effects of miRNA-215-5p on proliferative and metastatic capacities of LNCaP and DU-145 cells were evaluated through cell counting kit-8 (CCK-8) and transwell assay, respectively. Bioinformatics prediction was performed to search for the target genes of miRNA-215-5p and PGK1 was selected. The biological role of PGK1 in the progression of PCa was finally clarified by a series of rescue experiments.
MiRNA-215-5p was lowly expressed in PCa tissues and cell lines. Low level of miRNA-215-5p predicted poor prognosis in PCa patients. The silence of miRNA-215-5p enhanced viability, migratory, and invasive capacities of LNCaP cells, while the overexpression of miRNA-215-5p yielded the opposite trends in DU-145 cells. PGK1 was predicted to be the target of miRNA-215-5p. PGK1 was upregulated in PCa tissues and cell lines and its high level predicted poor prognosis of PCa. Moreover, PGK1 level was negatively correlated to that of miRNA-215-5p in PCa tissues. PGK1 was able to reverse the regulatory effects of miRNA-215-5p on metastatic potentials of PCa cells.
Downregulated miRNA-215-5p in PCa is closely related to distant metastasis and poor prognosis of affected patients. MiRNA-215-5p alleviates the malignant progression of PCa by targeting and downregulating PGK1.
微小 RNA（miRNAs）是内源性的非编码 RNA，在调控生物进程中发挥着关键作用。已有研究表明 miRNA-215-5p 具有抗肿瘤作用。然而，其影响前列腺癌（PCa）进展的具体作用尚不清楚。本研究旨在揭示 miRNA-215-5p 对 PCa 转移和预后的调控作用。
采用实时定量聚合酶链反应（qRT-PCR）检测 52 例 PCa 组织和 52 例癌旁组织中 miRNA-215-5p 的水平。分析 miRNA-215-5p 水平与 PCa 患者病理指标及总生存期的关系。通过细胞计数试剂盒-8（CCK-8）和 Transwell 实验分别评估 miRNA-215-5p 对 LNCaP 和 DU-145 细胞增殖和转移能力的调控作用。通过生物信息学预测寻找 miRNA-215-5p 的靶基因，并选择 PGK1。通过一系列挽救实验最终阐明 PGK1 在 PCa 进展中的生物学作用。
miRNA-215-5p 在 PCa 组织和细胞系中低表达。miRNA-215-5p 低表达预示着 PCa 患者预后不良。miRNA-215-5p 沉默增强了 LNCaP 细胞的活力、迁移和侵袭能力，而 DU-145 细胞中的过表达则呈现相反的趋势。PGK1 被预测为 miRNA-215-5p 的靶基因。PGK1 在 PCa 组织和细胞系中上调，其高水平预示着 PCa 的预后不良。此外，PGK1 水平与 PCa 组织中 miRNA-215-5p 水平呈负相关。PGK1 能够逆转 miRNA-215-5p 对 PCa 细胞转移潜能的调节作用。
PCa 中下调的 miRNA-215-5p 与受影响患者的远处转移和不良预后密切相关。miRNA-215-5p 通过靶向和下调 PGK1 减轻 PCa 的恶性进展。
