Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Cell Cycle. 2022 Jun;21(12):1316-1334. doi: 10.1080/15384101.2022.2050645. Epub 2022 Mar 15.
Since circCDR1 was abnormally expressed in esophageal squamous cell cancer (ESCC), the current study explored whether circCDR1 affected ESCC. Detailedly, circCDR1 expression in ESCC and linear isoform and stability of circCDR1 were detected by RT-qPCR. The location of circCDR1 was detected by fluorescence hybridization (FISH). After transfection, the cell biological functions were detected by wound-healing, CCK-8, colony formation, and flow cytometry assays. The target of circCDR1 was predicted by bioinformatics, FISH, RNA pull-down, and dual-luciferase reporter assays. The correlation between circCDR1 and miR-1290 was analyzed by Pearson's correlation analysis. A subcutaneous-xenotransplant tumor model in BALB/c nude mice was established and the levels of circCDR1, miR-1290, and apoptosis/metastasis/proliferation-related factors in the cancer cells and tissues were detected by immunohistochemical analysis, western blot, or RT-qPCR. As a result, circCDR1 was low-expressed in ESCC tissues and cells, while miR-1290 was high-expressed. CircCDR1 was regulated and was negatively correlated with miR-1290. CircCDR1, located in cytoplasm, inhibited the viability, proliferation, migration, and invasion of the cancer cells and the expressions of Bcl-2, N-cadherin, and Vimentin, but enhanced cell apoptosis and the expressions of C caspase-3, Bax, E-cadherin, IGFBP4, LHX6 and NFIX. , circCDR1 promoted xenotransplanted tumor weight and volume, and the expressions of C caspase-3 and Bax yet suppressed the levels of Bcl-2, miR-1290, and Ki-67 in tumor tissues. The effects of circCDR1 on both cancer cells and tissues were opposite to and reversed by miR-1290 mimic. Collectively, circCDR1 sponged miR-1290 to regulate the progression of ESCC both and .
由于 circCDR1 在食管鳞状细胞癌 (ESCC) 中异常表达，本研究探讨了 circCDR1 是否影响 ESCC。详细地，通过 RT-qPCR 检测 ESCC 中 circCDR1 的表达、线性异构体和 circCDR1 的稳定性。通过荧光杂交 (FISH) 检测 circCDR1 的位置。转染后，通过划痕愈合、CCK-8、集落形成和流式细胞术检测细胞生物学功能。通过生物信息学、FISH、RNA 下拉和双荧光素酶报告基因检测预测 circCDR1 的靶标。通过 Pearson 相关分析分析 circCDR1 与 miR-1290 的相关性。在 BALB/c 裸鼠中建立皮下异种移植肿瘤模型，并通过免疫组织化学分析、western blot 或 RT-qPCR 检测癌细胞和组织中 circCDR1、miR-1290 和凋亡/转移/增殖相关因子的水平。结果表明，circCDR1 在 ESCC 组织和细胞中低表达，而 miR-1290 高表达。circCDR1 受调控且与 miR-1290 呈负相关。circCDR1 位于细胞质中，抑制癌细胞的活力、增殖、迁移和侵袭，以及 Bcl-2、N-cadherin 和 Vimentin 的表达，但增强细胞凋亡和 C caspase-3、Bax、E-cadherin、IGFBP4、LHX6 和 NFIX 的表达。circCDR1 促进异种移植肿瘤的重量和体积，以及肿瘤组织中 C caspase-3 和 Bax 的表达，而抑制 Bcl-2、miR-1290 和 Ki-67 的水平。circCDR1 对癌细胞和组织的影响与 miR-1290 模拟相反且逆转。总之，circCDR1 作为 miR-1290 的海绵体调节 ESCC 的进展。
