Lucas-Torres Covadonga, Bernard Thierry, Huber Gaspard, Berthault Patrick, Nishiyama Yusuke, Kandiyal Pancham S, Elena-Herrmann Bénédicte, Molin Laurent, Solari Florence, Bouzier-Sore Anne-Karine, Wong Alan
NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191 Gif-sur-Yvette, France.
JEOL RESONANCE Inc., Musashino, Akishima, Tokyo 196-8558, Japan.
Metabolites. 2020 Jan 30;10(2):54. doi: 10.3390/metabo10020054.
The study of the metabolome within tissues, organisms, cells or biofluids can be carried out by several bioanalytical techniques. Among them, nuclear magnetic resonance (NMR) is one of the principal spectroscopic methods. This is due to a sample rotation technique, high-resolution magic angle spinning (HR-MAS), which targets the analysis of heterogeneous specimens with a bulk sample mass from 5 to 10 mg. Recently, a new approach, high-resolution micro-magic angle spinning (HR-μMAS), has been introduced. It opens, for the first time, the possibility of investigating microscopic specimens (<500 μg) with NMR spectroscopy, strengthening the concept of homogeneous sampling in a heterogeneous specimen. As in all bioanalytical approaches, a clean and reliable sample preparation strategy is a significant component in designing metabolomics (or -omics, in general) studies. The sample preparation for HR-μMAS is consequentially complicated by the μg-scale specimen and has yet to be addressed. This report details the strategies for three specimen types: biofluids, fluid matrices and tissues. It also provides the basis for designing future μMAS NMR studies of microscopic specimens.
可通过多种生物分析技术对组织、生物体、细胞或生物流体中的代谢组进行研究。其中，核磁共振（NMR）是主要的光谱方法之一。这得益于一种样品旋转技术，即高分辨率魔角旋转（HR-MAS），它针对的是质量为5至10毫克的大量样品的异质标本分析。最近，一种新方法——高分辨率微魔角旋转（HR-μMAS）被引入。它首次开启了用NMR光谱研究微观标本（<500μg）的可能性，强化了在异质标本中进行均匀采样的概念。与所有生物分析方法一样，清洁可靠的样品制备策略是设计代谢组学（或一般而言的组学）研究的重要组成部分。HR-μMAS的样品制备因微克级标本而变得复杂，目前尚未得到解决。本报告详细介绍了针对三种标本类型（生物流体、流体基质和组织）的策略。它还为设计未来对微观标本的μMAS NMR研究提供了基础。
