Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, 639 ZhiZaoJu Road, Shanghai, 200011, China.
Stem Cell Res Ther. 2020 Feb 4;11(1):50. doi: 10.1186/s13287-020-1564-7.
Tissue expansion techniques play an important role in plastic surgery. How to improve the quality of the expanded skin and shorten the expansion period are still worth investigating. Our previous studies found that a cell-free fat extract (CEFFE) possessed pro-angiogenic and pro-proliferative activities. However, the role of CEFFE on tissue expansion has remained unclear. The purpose of this study was to evaluate the effect of CEFFE on tissue expansion.
A rat tissue expansion model was used. Animals were treated with CEFFE by subcutaneous injection. After 4 weeks of tissue expansion, the skin necrosis and retraction rates were evaluated, the thicknesses of the epidermis and dermis were determined by histological analyses, blood vessel density was measured by anti-CD31 staining, cell proliferation was assessed by proliferating cell nuclear antigen staining, and the expression of specific proteins was evaluated by western blot analyses. In addition, the effects of CEFFE on the proliferation and cell cycle of cultured HaCaT cells were evaluated in vitro.
CEFFE treatment significantly decreased the necrosis rate and retraction of the expanded skin. The thickness of the epidermal and dermal layers was higher in CEFFE-treated compared to untreated skin. The density of blood vessels and cell proliferation in the epidermis of the expanded skin was improved by CEFFE treatment. In addition, CEFFE treatment significantly increased the expression of the vascular endothelial growth factor receptor, epidermal growth factor receptor, collagen type 1, and collagen type 3. CEFFE also increased the proliferation of HaCaT cells in culture.
CEFFE improves the quality of the expanded skin by promoting angiogenesis and cell proliferation. It could be potentially used clinically for augmenting tissue expansion.
组织扩张技术在整形外科学中具有重要作用。如何提高扩张皮肤的质量并缩短扩张周期仍值得研究。我们之前的研究发现，无细胞脂肪提取物（CEFFE）具有促血管生成和促增殖作用。然而，CEFFE 对组织扩张的作用仍不清楚。本研究旨在评估 CEFFE 对组织扩张的影响。
使用大鼠组织扩张模型。通过皮下注射向动物给予 CEFFE。在组织扩张 4 周后，评估皮肤坏死和回缩率，通过组织学分析测定表皮和真皮的厚度，通过抗 CD31 染色测量血管密度，通过增殖细胞核抗原染色评估细胞增殖，通过 Western blot 分析评估特定蛋白的表达。此外，还评估了 CEFFE 对体外培养的 HaCaT 细胞增殖和细胞周期的影响。
CEFFE 处理显著降低了扩张皮肤的坏死率和回缩率。与未处理皮肤相比，CEFFE 处理的表皮和真皮层厚度更高。CEFFE 处理可改善扩张皮肤表皮中的血管密度和细胞增殖。此外，CEFFE 处理还显著增加了血管内皮生长因子受体、表皮生长因子受体、胶原 I 型和胶原 III 型的表达。CEFFE 还增加了体外培养的 HaCaT 细胞的增殖。
CEFFE 通过促进血管生成和细胞增殖来改善扩张皮肤的质量。它可能有潜力在临床上用于增强组织扩张。
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