Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral and Craniofacial Sciences, King's College London, London, UK.
Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21, Stockholm, Sweden.
Nat Commun. 2020 Feb 4;11(1):693. doi: 10.1038/s41467-020-14422-w.
The global threat of antimicrobial resistance has driven the use of high-throughput sequencing techniques to monitor the profile of resistance genes, known as the resistome, in microbial populations. The human oral cavity contains a poorly explored reservoir of these genes. Here we analyse and compare the resistome profiles of 788 oral cavities worldwide with paired stool metagenomes. We find country and body site-specific differences in the prevalence of antimicrobial resistance genes, classes and mechanisms in oral and stool samples. Within individuals, the highest abundances of antimicrobial resistance genes are found in the oral cavity, but the oral cavity contains a lower diversity of resistance genes compared to the gut. Additionally, co-occurrence analysis shows contrasting ARG-species associations between saliva and stool samples. Maintenance and persistence of antimicrobial resistance is likely to vary across different body sites. Thus, we highlight the importance of characterising the resistome across body sites to uncover the antimicrobial resistance potential in the human body.
全球抗菌药物耐药性的威胁推动了高通量测序技术的应用，以监测微生物种群中耐药基因的特征，即耐药组。人类口腔中蕴藏着这些基因尚未被充分探索的宝库。在这里，我们分析和比较了全球 788 个口腔和配对粪便宏基因组的耐药组谱。我们发现，口腔和粪便样本中抗菌药物耐药基因、类别和机制的流行存在国家和身体部位特异性差异。在个体内部，口腔中发现的抗菌药物耐药基因丰度最高，但与肠道相比，口腔中的耐药基因多样性较低。此外，共现分析表明，唾液和粪便样本中 ARG-物种的关联存在差异。不同身体部位对抗菌药物耐药性的维持和持久性可能存在差异。因此，我们强调了在不同身体部位描述耐药组的重要性，以揭示人体中的抗菌药物耐药性潜力。
