Centre d'Etude et de Recherche sur le Paludisme Associé à la Grossesse et à l'Enfance, Faculté des Sciences de la Santé, Cotonou, Bénin.
UMR 261 MERIT, Université de Paris, Institut de Recherche pour le Développement (IRD), Paris, France.
Parasite Immunol. 2020 Apr;42(4):e12702. doi: 10.1111/pim.12702. Epub 2020 Feb 24.
Schistosomiasis and malaria are endemic in sub-Saharan Africa where Schistosoma haematobium (Sh) and Plasmodium falciparum (Pf) coinfections are thus frequent. We explored the effect of Sh infection on antibody responses directed to Pf merozoite antigens and on malaria susceptibility in Beninese children.
A total of 268 children were followed during a malaria transmission season. Detection of Pf infection was performed by microscopy and rapid diagnostic tests. Sh infection was determined in urine by microscopy. Antimalarial antibody, cytokine and HLA-G concentrations were quantified by ELISA. The expression of HLA-G receptors by immune cells was assessed by flow cytometry. Children infected by Sh had higher concentrations of IgG1 directed to MSP3 and GLURP , IgG2 directed to GLURP and IgG3 directed to MSP3, GLURP and GLURP and have lower Pf densities than those uninfected by Sh. No difference in cytokine and HLA-G concentrations was observed between Sh egg carriers and non-carriers.
Schistosoma haematobium modulates host immune responses directed to Pf antigens. The absence of immune downregulation usually observed during helminth infections is surprising in our study. We hypothesize that the stage of Sh development could partly explain the immune pathways leading to increased antibody levels that favour better control of Pf parasitemia.
在撒哈拉以南非洲，血吸虫病和疟疾流行，因此，曼氏血吸虫（Sh）和恶性疟原虫（Pf）混合感染很常见。本研究旨在探讨 Sh 感染对 Pf 裂殖子抗原抗体反应和儿童疟疾易感性的影响。
本研究共纳入 268 名儿童，在疟疾传播季节进行了随访。通过显微镜检查和快速诊断检测来检测 Pf 感染，通过显微镜检查尿液来检测 Sh 感染。通过 ELISA 定量检测抗疟抗体、细胞因子和 HLA-G 的浓度，通过流式细胞术评估免疫细胞 HLA-G 受体的表达。与未感染 Sh 的儿童相比，感染 Sh 的儿童对 MSP3 和 GLURP 的 IgG1、对 GLURP 的 IgG2 和对 MSP3、GLURP 和 GLURP 的 IgG3 的浓度更高，Pf 密度更低。Sh 卵携带者和非携带者之间细胞因子和 HLA-G 浓度无差异。
曼氏血吸虫调节宿主针对 Pf 抗原的免疫反应。在本研究中，我们观察到通常在寄生虫感染中观察到的免疫下调缺失，这令人惊讶。我们假设 Sh 发育的阶段部分解释了导致抗体水平升高的免疫途径，这有利于更好地控制 Pf 寄生虫血症。
