1型糖尿病患者循环中组织因子阳性促凝微粒
Circulating Tissue Factor-Positive Procoagulant Microparticles in Patients with Type 1 Diabetes.
作者信息
Zhang Chenghui, Ou Qing, Gu Yan, Cheng Gaiping, Du Rong, Yuan Li, Cordiner Ruth Lm, Kang Deying, Zhang Jiaying, Huang Qiaorong, Yu Chuan, Kang Li, Wang Xuan, Sun Xin, Mo Xianming, Tian Haoming, Pearson Ewan R, Meng Wentong, Li Sheyu
机构信息
Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
Department of Endocrinology and Metabolism, Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Chengdu 610041, People's Republic of China.
出版信息
Diabetes Metab Syndr Obes. 2019 Dec 31;12:2819-2828. doi: 10.2147/DMSO.S225761. eCollection 2019.
AIM
To investigate the count of circulating tissue factor-positive (TF) procoagulant microparticles (MPs) in patients with type 1 diabetes mellitus (T1DM).
METHODS
This case-control study included patients with T1DM and age and sex-matched healthy volunteers. The counts of phosphatidylserine-positive (PS) MPs and TFPSMPs and the subgroups derived from different cell types were measured in the peripheral blood sample of the two groups using multicolor flow cytometric assay. We compared the counts of each MP between groups as well as the ratio of the TFPSMPs and PSMPs (TFPSMPs/PSMPs).
RESULTS
We recruited 36 patients with T1DM and 36 matched healthy controls. Compared with healthy volunteers, PSMPs, TFPSMPs and TFPSMPs/PSMPs were elevated in patients with T1DM (PSMPs: 1078.5 ± 158.08 vs 686.84 ± 122.04/μL, <0.001; TFPSMPs: 202.10 ± 47.47 vs 108.33 ± 29.42/μL, <0.001; and TFPSMPs/PSMPs: 0.16 ± 0.04 vs 0.19 ± 0.05, P = 0.004), mostly derived from platelet, lymphocytes and endothelial cells. In the subgroup analysis, the counts of total and platelet TFPSMPs were increased in patients with diabetic retinopathy (DR) and with higher HbA1c, respectively.
CONCLUSION
Circulating TFPSMPs and those derived from platelet, lymphocytes and endothelial cells were elevated in patients with T1DM.
目的
研究1型糖尿病（T1DM）患者循环组织因子阳性（TF）促凝微粒（MPs）的数量。
方法
本病例对照研究纳入了T1DM患者以及年龄和性别匹配的健康志愿者。使用多色流式细胞术检测两组外周血样本中磷脂酰丝氨酸阳性（PS）MPs、TFPSMPs以及源自不同细胞类型的亚组数量。我们比较了两组之间每种MP的数量以及TFPSMPs与PSMPs的比例（TFPSMPs/PSMPs）。
结果
我们招募了36例T1DM患者和36例匹配的健康对照。与健康志愿者相比，T1DM患者的PSMPs、TFPSMPs和TFPSMPs/PSMPs升高（PSMPs：1078.5±158.08对686.84±122.04/μL，<0.001；TFPSMPs：202.10±47.47对108.33±29.42/μL，<；0.001；TFPSMPs/PSMPs：0.16±0.04对0.19±0.05，P = 0.004），主要源自血小板、淋巴细胞和内皮细胞。在亚组分析中，糖尿病视网膜病变（DR）患者和糖化血红蛋白（HbA1c）较高的患者中，总TFPSMPs和血小板TFPSMPs的数量分别增加。
结论
T1DM患者循环中的TFPSMPs以及源自血小板、淋巴细胞和内皮细胞的TFPSMPs升高。
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