Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark.
Biomolecular Nanoscale Engineering Center, Department of Chemistry, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.
Chemistry. 2020 May 4;26(25):5676-5684. doi: 10.1002/chem.201905636. Epub 2020 Apr 17.
DNA nanostructures have been designed and used in many different applications. However, the use of nucleic acid scaffolds to promote the self-assembly of artificial protein mimics is only starting to emerge. Herein five coiled-coil peptide structures were templated by the hybridization of a d-DNA triplex or its mirror-image counterpart, an l-DNA triplex. The self-assembly of the desired trimeric structures in solution was confirmed by gel electrophoresis and small-angle X-ray scattering, and the stabilizing synergy between the two domains was found to be chirality-independent but orientation-dependent. This is the first example of using a nucleic acid scaffold of l-DNA to template the formation of artificial protein mimics. The results may advance the emerging POC-based nanotechnology field by adding two extra dimensions, that is, chirality and polarity, to provide innovative molecular tools for rational design and bottom-up construction of artificial protein mimics, programmable materials and responsive nanodevices.
DNA 纳米结构已被设计并应用于许多不同的领域。然而，利用核酸支架来促进人工蛋白模拟物的自组装才刚刚开始出现。在这里，五个卷曲螺旋肽结构通过 d-DNA 三螺旋或其镜像 l-DNA 三螺旋的杂交进行模板化。通过凝胶电泳和小角 X 射线散射确认了所需三聚体结构在溶液中的自组装，并且发现两个结构域之间的稳定协同作用是与手性无关但与取向有关。这是首次使用 l-DNA 核酸支架来模板化人工蛋白模拟物形成的实例。研究结果可能会通过为人工蛋白模拟物、可编程材料和响应性纳米器件的理性设计和自下而上构建提供创新的分子工具，为新兴的基于 POCT 的纳米技术领域增添两个额外的维度，即手性和极性，从而推动该领域的发展。
