College of Medicine, China Medical University, Taichung, Taiwan.
Department of Emergency Medicine, China Medical University Hospital, Taichung, Taiwan.
Environ Toxicol. 2020 Jun;35(6):707-713. doi: 10.1002/tox.22906. Epub 2020 Feb 5.
Excessive intake of high fat diet (HFD) and associated obese conditions are critical contributors of cardiac diseases. In this study, an active metabolite andrographolide from Andrographis paniculata was found to ameliorate HFD-induced cardiac apoptosis. C57/BL6 mouse were grouped as control (n = 9), obese (n = 8), low dose (25 mg/kg/d) andrographolide treatment (n = 9), and high dose (50 mg/kg/d) andrographolide treatment (n = 9). The control group was provided with standard laboratory chow and the other groups were fed with HFD. Andrographolide was administered through oral gavage for 1 week. Histopathological analysis showed increase in apoptotic nuclei and considerable cardiac-damages in the obese group signifying cardiac remodeling effects. Further, Western blot results showed increase in pro-apoptotic proteins and decrease in the proteins of IGF-1R-survival signaling. However, feeding of andrographolide significantly reduced the cardiac effects of HFD. The results strongly suggest that andrographolide supplementation can be used for prevention and treatment of cardiovascular disease in obese patients.
过量摄入高脂肪饮食（HFD）和相关的肥胖状况是心脏疾病的重要致病因素。在这项研究中，发现穿心莲中的一种活性代谢物穿心莲内酯可改善 HFD 诱导的心脏细胞凋亡。将 C57/BL6 小鼠分为对照组（n = 9）、肥胖组（n = 8）、低剂量（25mg/kg/d）穿心莲内酯治疗组（n = 9）和高剂量（50mg/kg/d）穿心莲内酯治疗组（n = 9）。对照组给予标准实验室饲料，其他组给予 HFD。通过口服灌胃给予穿心莲内酯治疗 1 周。组织病理学分析显示肥胖组的凋亡核增加，心脏损伤明显，表明存在心脏重塑作用。此外，Western blot 结果显示促凋亡蛋白增加，IGF-1R 存活信号蛋白减少。然而，穿心莲内酯的喂养显著减轻了 HFD 的心脏作用。这些结果强烈表明，穿心莲内酯的补充可用于预防和治疗肥胖患者的心血管疾病。
