Brągoszewska Ewa, Biedroń Izabela, Hryb Wojciech
Faculty of Power and Environmental Engineering, Department of Technologies and Installations for Waste Management, Silesian University of Technology, 18 Konarskiego St., 44-100 Gliwice, Poland.
Institute for Ecology of Industrial Areas, Environmental Microbiology Unit, 6 Kossutha St., 40-844 Katowice, Poland.
Microorganisms. 2020 Jan 31;8(2):202. doi: 10.3390/microorganisms8020202.
International interests in biological air pollutants have increased rapidly to broaden the pool of knowledge on their identification and health impacts (e.g., infectious, respiratory diseases and allergies). Antibiotic resistance and its wider implications present us with a growing healthcare crisis, and an increased understanding of antibiotic-resistant bacteria populations should enable better interpretation of bioaerosol exposure found in the air. Waste sorting plant (WSP) activities are a source of occupational bacterial exposures that are associated with many health disorders. The objectives of this study were (a) to assess bacterial air quality (BAQ) in two cabins of a WSP: preliminary manual sorting cabin (PSP) and purification manual sorting cabin (quality control) (QCSP), (b) determine the particle size distribution (PSD) of bacterial aerosol (BA) in PSP, QCSP, and in the outdoor air (OUT), and (c) determine the antibiotic resistance of isolated strains of bacteria. Bacterial strains were identified on a Biolog GEN III (Biolog, Hayward, CA, USA), and disc diffusion method for antimicrobial susceptibility testing was carried out according to the Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. A large share of fecal bacteria, and spp. was found in the tested indoor air which is a potential health hazard to the workers of the monitored WSP. Our results demonstrate the necessity to take into account fecal air pollution levels to avoid making erroneous assumptions regarding the environmental selection of antibiotic resistance. Total elimination of many anthropogenic sources is not possible, but important findings of this study can be used to develop realistic management policies methods to improve BAQ.
国际上对生物空气污染物的关注迅速增加，以拓宽关于其识别和健康影响（如传染病、呼吸道疾病和过敏）的知识储备。抗生素耐药性及其更广泛的影响给我们带来了日益严重的医疗危机，而对抗生素耐药细菌群体的更多了解应有助于更好地解读空气中发现的生物气溶胶暴露情况。垃圾分类处理厂（WSP）的活动是职业性细菌暴露的一个来源，与许多健康问题相关。本研究的目的是：（a）评估一个WSP的两个工作间的细菌空气质量（BAQ）：初步人工分拣间（PSP）和净化人工分拣间（质量控制）（QCSP）；（b）确定PSP、QCSP和室外空气（OUT）中细菌气溶胶（BA）的粒径分布（PSD）；（c）确定分离出的细菌菌株的抗生素耐药性。在Biolog GEN III（美国加利福尼亚州海沃德市的Biolog公司）上鉴定细菌菌株，并根据柯氏-鲍氏纸片扩散药敏试验方案进行纸片扩散法抗菌药敏试验。在测试的室内空气中发现了很大比例的粪便细菌和 属细菌，这对受监测的WSP的工人构成潜在健康危害。我们的结果表明，有必要考虑粪便空气污染水平，以避免在抗生素耐药性的环境选择方面做出错误假设。完全消除许多人为来源是不可能的，但本研究的重要发现可用于制定切实可行的管理政策和方法，以改善BAQ。
