West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, P.O. Box LG 25, Legon, Accra, Ghana.
Department of Biochemistry, Cell & Molecular Biology, University of Ghana, P.O. Box LG 54, Legon, Accra, Ghana.
Sensors (Basel). 2020 Feb 1;20(3):799. doi: 10.3390/s20030799.
The impact of malaria on global health has continually prompted the need to develop more effective diagnostic strategies that could overcome deficiencies in accurate and early detection. In this review, we examine the various biosensor-based methods for malaria diagnostic biomarkers, namely; histidine-rich protein 2 (PfHRP-2), parasite lactate dehydrogenase (pLDH), aldolase, glutamate dehydrogenase (GDH), and the biocrystal hemozoin. The models that demonstrate a potential for field application have been discussed, looking at the fabrication and analytical performance characteristics, including (but not exclusively limited to): response time, sensitivity, detection limit, linear range, and storage stability, which are first summarized in a tabular form and then described in detail. The conclusion summarizes the state-of-the-art technologies applied in the field, the current challenges and the emerging prospects for malaria biosensors.
疟疾对全球健康的影响不断促使人们开发更有效的诊断策略，以克服准确和早期检测的不足。在这篇综述中，我们研究了各种基于生物传感器的疟疾诊断生物标志物方法，即：富含组氨酸蛋白 2（PfHRP-2）、寄生虫乳酸脱氢酶（pLDH）、醛缩酶、谷氨酸脱氢酶（GDH）和生物晶体血晶素。讨论了具有潜在现场应用前景的模型，考察了制造和分析性能特征，包括（但不限于）：响应时间、灵敏度、检测限、线性范围和存储稳定性，这些特征首先以表格形式总结，然后详细描述。结论总结了该领域应用的最新技术、当前的挑战以及疟疾生物传感器的新兴前景。
