Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781039 Assam, India.
Centre for Biosensors, Bioelectronics and Biodevices (C3Bio) and Department of Electronic and Electrical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom.
Biosens Bioelectron. 2018 Oct 15;117:246-252. doi: 10.1016/j.bios.2018.06.022. Epub 2018 Jun 8.
A capacitive aptasensor for detecting the malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (PfGDH), directly in human serum samples developed. A thiolated ssDNA aptamer (NG3) that binds specifically to PfGDH antigen with high affinity (K= 79 nM) was used to develop the aptasensor. The aptasensor produced capacitance response at an optimized frequency of 2 Hz in a non-Faradaic electrochemical impedance based signal transduction platform. The aptasensor exhibited a wide dynamic range of 100 fM-100 nM with a limits of detection of 0.77 pM in serum samples. The interference from other predominant malarial biomarkers, namely, Plasmodium falciparum -lactate dehydrogenase and -histidine rich protein-II on the aptasensor was negligible. This PfGDH aptasensor with highly sensitive and label free detection capability has great application potential for diagnosis of asymptotic malaria and monitoring the regression of malaria during treatment regime with antimalarial drugs.
一种用于直接检测人血清样本中疟疾生物标志物恶性疟原虫谷氨酸脱氢酶（PfGDH）的电容适体传感器得以开发。该适体传感器使用与 PfGDH 抗原具有高亲和力（K=79 nM）特异性结合的硫醇化 ssDNA 适体（NG3）构建。在基于非法拉第电化学阻抗的信号转导平台中，该适体传感器在优化频率 2 Hz 下产生电容响应。该适体传感器在血清样本中具有 100 fM-100 nM 的宽动态范围和 0.77 pM 的检测限。其他主要的疟疾生物标志物，即恶性疟原虫乳酸脱氢酶和富含组氨酸蛋白-II 对适体传感器的干扰可以忽略不计。这种具有高灵敏度和无标记检测能力的 PfGDH 适体传感器在无症状疟疾的诊断和抗疟药物治疗期间疟疾消退的监测方面具有巨大的应用潜力。
