Biotropics Malaysia Berhad, Lot 21 Jalan U1/19, Section U1Hicom Glenmarie Industrial Park, Shah Alam, Selangor, Malaysia.
Etica Clinpharm Pvt. Ltd, Raipur, Chhattisgarh, 492001, India.
BMC Complement Med Ther. 2020 Feb 5;20(1):31. doi: 10.1186/s12906-020-2814-z.
Each year 1.5 million women experience menopause when menstrual cycles cease resulting from the loss of ovarian function and oestrogen deprivation, a hormone that helps prevent bone loss. This study investigated the effects of Physta®, a standardized herbal extract of Eurycoma longifolia Jack (PEL), on hormonal balance and parameters associated with hormonal imbalance, namely body and uterus weight and bone biochemical markers relevant in menopausal symptoms.
Forty-eight Sprague Dawley rats were randomly divided into six groups of eight rats each: (A) Sham operated; control (B) Untreated (ovariectomised (OVX) with vehicle), (C) PEL 100 (OVX + 100 mg/kg body weight (bw)), (D) PEL 300 (OVX + 300 mg/kg bw), (E) PEL 500 (OVX + 500 mg/kg bw) and (F) Positive control, testosterone undecanoate (TU) (OVX+ 10 mg/kg bw). Group A and B received daily oral administrations of the vehicle, Group C-E received daily oral administration of PEL and Group F received testosterone undecanoate intramuscularly weekly. At the end of 8 weeks, serum calcium, phosphate, bone alkaline phosphatase (BALP), osteocalcin, follicle stimulating hormone (FSH), luteinising hormone (LH), oestrogen, progesterone and testosterone were measured, then the animals were sacrificed and uterus was isolated, while weight was recorded in all experimental groups.
Treatment of OVX rats with PEL at a dose of 500 mg/kg showed decreased serum FSH (P < 0.001, 4.25 ± 0.22 mIU/ml) and LH (NS, 4.07 ± 0.12 mIU/ml), while there was a significant increase in progesterone (P < 0.05, 2.48 ± 0.08 ng/ml) and oestrogen (P < 0.05, 11.02 ± 0.13 pg/ml) levels when compared to untreated group. PEL treatment at doses of 100 mg/kg, 300 mg/kg and 500 mg/kg showed a non-significant but increasing trend in serum calcium, phosphate, bone alkaline phosphate and testosterone levels. Ovariectomy resulted in a significant reduction (P < 0.001, 238.81 ± 5.39 mg) in uterus weight in the ovariectomised rats, which was alleviated in all PEL treated ovariectomised rats with an increasing trend of uterine weight.
The results suggest that PEL could be protective and beneficial for the management of reproductive hormone and bone markers. Therefore, it could be used to address hormonal imbalances and symptoms associated with menopause.
每年有 150 万名女性经历绝经，这是由于卵巢功能丧失和雌激素剥夺导致的月经周期停止，雌激素是一种有助于预防骨质流失的激素。本研究旨在调查 Physta®（Eurycoma longifolia Jack 的标准化草药提取物）对激素平衡和与激素失衡相关的参数的影响，即身体和子宫重量以及与绝经症状相关的骨生化标志物。
将 48 只 Sprague Dawley 大鼠随机分为六组，每组 8 只：（A）假手术；对照（B）未治疗（卵巢切除术，给予载体），（C）PEL100（卵巢切除+100mg/kg 体重），（D）PEL300（卵巢切除+300mg/kg 体重），（E）PEL500（卵巢切除+500mg/kg 体重）和（F）阳性对照，十一酸睾酮（TU）（卵巢切除+10mg/kg 体重）。A 组和 B 组每日口服给予载体，C-E 组每日口服给予 PEL，F 组每周肌肉内给予十一酸睾酮。8 周后，测量血清钙、磷、骨碱性磷酸酶（BALP）、骨钙素、卵泡刺激素（FSH）、黄体生成素（LH）、雌激素、孕酮和睾酮，然后处死动物并分离子宫，同时记录所有实验组的子宫重量。
卵巢切除大鼠给予 PEL 500mg/kg 剂量治疗时，血清 FSH 降低（P<0.001，4.25±0.22 mIU/ml）和 LH 无显著差异（NS，4.07±0.12 mIU/ml），而孕酮（P<0.05，2.48±0.08ng/ml）和雌激素（P<0.05，11.02±0.13pg/ml）水平显著升高。PEL 100mg/kg、300mg/kg 和 500mg/kg 剂量治疗时，血清钙、磷、骨碱性磷酸酶和睾酮水平呈非显著但逐渐升高趋势。卵巢切除术导致子宫重量显著降低（P<0.001，238.81±5.39mg），所有 PEL 治疗的卵巢切除大鼠的子宫重量均得到缓解，并呈子宫重量增加的趋势。
结果表明，PEL 可能对生殖激素和骨标志物具有保护和有益作用。因此，它可用于解决与绝经相关的激素失衡和症状。
