使用 3D 脑类器官研究人类神经发育和疾病。
Studying Human Neurodevelopment and Diseases Using 3D Brain Organoids.
机构信息
Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.
Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada,
出版信息
J Neurosci. 2020 Feb 5;40(6):1186-1193. doi: 10.1523/JNEUROSCI.0519-19.2019.
differentiation of pluripotent stem cells provides a systematic platform to study development and disease. Recent advances in brain organoid technology have created new opportunities to investigate the formation and function of the human brain, under physiological and pathological conditions. Brain organoids can be generated to model the cellular and structural development of the human brain, and allow the investigation of the intricate interactions between resident neural and glial cell types. Combined with new advances in gene editing, imaging, and genomic analysis, brain organoid technology can be applied to address questions pertinent to human brain development, disease, and evolution. However, the current iterations of brain organoids also have limitations in faithfully recapitulating the processes. In this perspective, we evaluate the recent progress in brain organoid technology, and discuss the experimental considerations for its utilization. Integrating CRISPR Engineering and hiPSC-Derived 2D Disease Modeling Systems, by Kristina Rehbach, Michael B. Fernando, and Kristen J. Brennand.
多能干细胞的分化为研究发育和疾病提供了一个系统的平台。最近，脑类器官技术的进步为在生理和病理条件下研究人脑的形成和功能创造了新的机会。脑类器官可以用来模拟人脑的细胞和结构发育，并允许研究驻留的神经和神经胶质细胞类型之间的复杂相互作用。结合基因编辑、成像和基因组分析方面的新进展，脑类器官技术可用于解决与人类大脑发育、疾病和进化相关的问题。然而，目前的脑类器官在忠实再现这些过程方面也存在局限性。在这篇观点文章中，我们评估了脑类器官技术的最新进展，并讨论了其应用的实验注意事项。整合 CRISPR 工程和 hiPSC 衍生的 2D 疾病建模系统，作者：Kristina Rehbach、Michael B. Fernando 和 Kristen J. Brennand。
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