Department of Oncology, Military Institute of Medicine, Warsaw, Poland.
Mazovian Center for Lung Diseases and Tuberculosis, Division III in Otwock, Otwock, Poland.
Sci Rep. 2020 Feb 5;10(1):1871. doi: 10.1038/s41598-020-58777-y.
Gallbladder cancer (GBC) is a highly malignant tumor with poorly understood etiology. An insight into phenotypic features of this malignancy may add to the knowledge of its carcinogenesis and pave the way to new therapeutic approaches. We assessed the expression of female sex hormone receptors (ERα, ERβ, PR), connective tissue growth factor (CTGF) and HER2 in GBC, and adjacent normal tissue (NT), and determined their prognostic impact. Immunohistochemical (IHC) expression of all biomarkers was performed in formalin-fixed, paraffin-embedded specimens in 60 Caucasian GBC patients (51 women and 9 men). ERβ, cytoPR and CTGF expression were found in 89%, 27%, 91% of GBC, and in 63%, 87%, 100% of NT, respectively. No ERα expression was found in GBC and NT. Strong (3+) HER2 expression by IHC or HER2 amplification was seen in five GBC (10.4%). A positive correlation was found between HER2 and CTGF and ERβ expression in GBC and matched NT. In the multivariate analysis, patient age >70 years, tumor size and ERβ expression in GBC was highly predictive for OS (p = 0.003). The correlation between HER2, CTGF and ERβ expression in GBC and NT may indicate the interaction of these pathways in physiological processes and gallbladder pathology.
胆囊癌（GBC）是一种恶性程度很高的肿瘤，其病因尚不清楚。深入了解这种恶性肿瘤的表型特征可能有助于了解其致癌机制，并为新的治疗方法铺平道路。我们评估了女性性激素受体（ERα、ERβ、PR）、结缔组织生长因子（CTGF）和 HER2 在 GBC 及相邻正常组织（NT）中的表达，并确定了它们的预后影响。在 60 名高加索 GBC 患者（51 名女性和 9 名男性）的福尔马林固定、石蜡包埋标本中进行了所有生物标志物的免疫组织化学（IHC）表达分析。在 GBC 中发现 ERβ、细胞 PR 和 CTGF 的表达分别为 89%、27%、91%，在 NT 中分别为 63%、87%、100%。在 GBC 和 NT 中均未发现 ERα 表达。IHC 或 HER2 扩增显示 5 例 GBC（10.4%）存在强（3+）HER2 表达。在 GBC 和匹配的 NT 中，HER2 与 CTGF 和 ERβ 表达之间存在正相关。在多变量分析中，患者年龄>70 岁、肿瘤大小和 GBC 中的 ERβ 表达是 OS 的高度预测因素（p=0.003）。GBC 和 NT 中 HER2、CTGF 和 ERβ 表达之间的相关性可能表明这些途径在生理过程和胆囊病理中的相互作用。
