Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Yanjing Medical College, Capital Medical University, Beijing, 100069, China.
Int Urol Nephrol. 2020 Jun;52(6):1049-1055. doi: 10.1007/s11255-019-02372-2. Epub 2020 Feb 5.
Genistein is recognized as a potent anti-oxidant in soybean-enriched foods, which is a kind of phytoestrogen involved in anticancer activity in various cancers.
The objective of this study was to investigate the molecular mechanism of CDKN2a hypomethylation involved in the anti-tumor effect of genistein on kidney cancer.
The CDKN2a expression was measured using qRT-PCR. The level of CDKN2a methylation was detected using methylation-specific PCR. The apoptosis was detected via flow-cytometric analysis. The cell viability was detected using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Our results indicated that genistein induced cell apoptosis and inhibited the cell proliferation of kidney cancer cells. Moreover, genistein increased the expression of CDKN2a and decreased CDKN2a methylation.
Our results demonstrated that the anti-tumor effect of genistein might induce cell apoptosis and inhibit the proliferation of kidney cancer cells via regulating CDKN2a methylation.
大豆中富含的染料木黄酮被认为是一种强效抗氧化剂，它是一种植物雌激素，参与多种癌症的抗癌活性。
本研究旨在探讨染料木黄酮抑制 CDKN2a 低甲基化的分子机制，及其对肾癌的抗肿瘤作用。
采用 qRT-PCR 检测 CDKN2a 的表达。采用甲基化特异性 PCR 检测 CDKN2a 的甲基化水平。采用流式细胞术检测细胞凋亡。采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法检测细胞活力。
我们的结果表明，染料木黄酮诱导了肾癌细胞的细胞凋亡并抑制了其增殖。此外，染料木黄酮增加了 CDKN2a 的表达并降低了 CDKN2a 的甲基化水平。
我们的结果表明，染料木黄酮的抗肿瘤作用可能通过调节 CDKN2a 甲基化来诱导细胞凋亡并抑制肾癌细胞的增殖。
