Faculté de Médecine Dentaire de Monastir, Unité des Substances Naturells Bioactives et Biotechnologie, Université de Monastir, Rue Avicenne, U17ES49, 5000, Monastir, Tunisia.
Institut supérieur des sciences appliquées et de technologie de Gabès, Université de Gabès, Avenue Omar Ibn El Khattab, Zrig Eddakhlania, 6029, Gabès, Tunisia.
Environ Sci Pollut Res Int. 2020 Apr;27(12):13417-13427. doi: 10.1007/s11356-020-07900-7. Epub 2020 Feb 5.
Cisplatin is an effective chemotherapeutic agent that has pronounced adverse effects. Using flavonoids is currently eliciting considerable interest. During extraction and conditioning, they usually undergo several physical treatments such as heat treatment, although it is not known whether thermal treatment might influence the pharmacological effects of flavonoids such as luteolin-7-O-glucoside (L7G). This study was undertaken to explore the protective role of native and heated L7G against DNA damage and oxidative stress induced by cisplatin. Balb/c mice were administered L7G before a single intraperitoneal injection of cisplatin (10 mg/kg). Animals were sacrificed 24 h after treatment with drugs. The geno-protective role of native and heated L7G was evaluated by comet assay. In addition to monitoring the activities of antioxidant enzymes, levels of malondialdehyde and reduced glutathione were assessed in the liver, kidney, brain, and spleen tissues. The results of the present study demonstrate that both heated and native L7G, at a dose of 40 mg/kg b.w, were able to reduce the genotoxicity of cisplatin. They attenuate the oxidative stress (malondialdehyde, catalase, GPx, SOD, and GSH) and tissue damage (creatinine, IFNγ). Heat treatment did not alter the antigenotoxic effect observed for native L7G and showed similar effects to those of native L7G for all of the evaluated parameters. Our study reveals that L7G attenuates the side effects of anticancer drug and heat treatment did not alter his antigenotoxic and antioxidant the potential.
顺铂是一种有效的化疗药物，具有明显的不良反应。目前，使用类黄酮引起了相当大的兴趣。在提取和调理过程中，它们通常会经历几次物理处理，如热处理，尽管尚不清楚热处理是否会影响类黄酮如木犀草素-7-O-葡萄糖苷（L7G）的药理作用。本研究旨在探讨天然和加热的 L7G 对顺铂诱导的 DNA 损伤和氧化应激的保护作用。Balb/c 小鼠在单次腹腔注射顺铂（10mg/kg）前给予 L7G。用药物处理 24 小时后处死动物。通过彗星试验评估天然和加热的 L7G 的遗传保护作用。除了监测抗氧化酶的活性外，还评估了肝、肾、脑和脾组织中丙二醛和还原型谷胱甘肽的水平。本研究结果表明，在 40mg/kg b.w 的剂量下，加热和天然 L7G 均能降低顺铂的遗传毒性。它们减轻氧化应激（丙二醛、过氧化氢酶、GPx、SOD 和 GSH）和组织损伤（肌酐、IFNγ）。热处理没有改变天然 L7G 观察到的抗原毒性作用，并对所有评估参数表现出与天然 L7G 相似的作用。我们的研究表明，L7G 减轻了抗癌药物的副作用，热处理没有改变其抗原毒性和抗氧化潜力。
