Toxicology and Biomedicine Research Group, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
Division of Hematology and Oncology, Department of Medicine, Changhua Christian Hospital, Changhua 500, Taiwan.
Biomolecules. 2020 Mar 26;10(4):502. doi: 10.3390/biom10040502.
Oral squamous cell carcinoma is the sixth most common type of cancer globally, which is associated with high rates of cancer-related deaths. Metastasis to distant organs is the main reason behind worst prognostic outcome of oral cancer. In the present study, we aimed at evaluating the effects of a natural plant flavonoid, luteolin-7-O-glucoside, on oral cancer cell migration and invasion. The study findings showed that in addition to preventing cell proliferation, luteolin-7-O-glucoside caused a significant reduction in oral cancer cell migration and invasion. Mechanistically, luteolin-7-O-glucoside caused a reduction in cancer metastasis by reducing p38 phosphorylation and downregulating matrix metalloproteinase (MMP)-2 expression. Using a p38 inhibitor, SB203580, we proved that luteolin-7-O-glucoside exerts anti-migratory effects by suppressing p38-mediated increased expression of MMP-2. This is the first study to demonstrate the luteolin-7-O-glucoside inhibits cell migration and invasion by regulating MMP-2 expression and extracellular signal-regulated kinase pathway in human oral cancer cell. The study identifies luteolin-7-O-glucoside as a potential anti-cancer candidate that can be utilized clinically for improving oral cancer prognosis.
口腔鳞状细胞癌是全球第六大常见癌症类型，与癌症相关的死亡率很高。远处器官转移是口腔癌预后最差的主要原因。在本研究中，我们旨在评估天然植物类黄酮木樨草素-7-O-葡萄糖苷对口腔癌细胞迁移和侵袭的影响。研究结果表明，木樨草素-7-O-葡萄糖苷除了预防细胞增殖外，还显著降低了口腔癌细胞的迁移和侵袭。在机制上，木樨草素-7-O-葡萄糖苷通过减少 p38 磷酸化和下调基质金属蛋白酶（MMP）-2 的表达来减少癌症转移。使用 p38 抑制剂 SB203580，我们证明木樨草素-7-O-葡萄糖苷通过抑制 p38 介导的 MMP-2 表达增加来发挥抗迁移作用。这是第一项证明木樨草素-7-O-葡萄糖苷通过调节 MMP-2 表达和细胞外信号调节激酶途径抑制人口腔癌细胞迁移和侵袭的研究。该研究确定木樨草素-7-O-葡萄糖苷是一种有潜力的抗癌候选药物，可用于临床改善口腔癌预后。
