College of Pharmaceutical Sciences, Yunnan University of Traditional Chinese Medicine, Kunming, People's Republic of China.
Guangdong Key Laboratory for Genome Stability & Disease Prevention, School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen, People's Republic of China.
J Appl Microbiol. 2021 Feb;130(2):592-603. doi: 10.1111/jam.14606. Epub 2020 Feb 27.
Diseases caused by pathogenic fungi was a major constrain in increasing productivity and improving quality of Panax notoginseng. The aim of this research was to evaluate the inhibitory activity of essential oils (EOs) from Asteraceae family, Chrysanthemum indicum and Laggera pterodonta, against pathogenic fungi of P. notoginseng.
The antifungal activity was investigated using multiple methods, disclosing that the EOs from C. indicum and L. pterodonta are active against hypha growth of different fungi but with different degrees of potency. Checkerboard testing indicated that the combination of EOs with hymexazol had synergistic effect against Pythium aphanidermatum, and exhibited additive effects against bulk of targeted pathogenic fungi. Besides, we found that the baseline sensitivity of Fusarium oxysporum to L. pterodonta EOs was higher than those of C. indicum by means of mycelium growth rate method. Finally, the practicability of those EOs as plant pesticide was confirmed by in vivo model showing that EOs can significantly inhibit the occurrence of root rot of P. notoginseng caused by F. oxysporum.
Those studies suggest that the EOs from C. indicum and L. pterodonta had the potential to develop into new pollution-free pesticides for the protection of precious Chinese herbal medicines.
This study provided a new way of biological control for overcoming the frequent diseases occurrence of P. notoginseng.
致病真菌引起的疾病是提高三七产量和改善三七质量的主要制约因素。本研究旨在评估菊科植物菊花和Laggera pterodonta 的精油（EOs）对三七病原菌的抑制活性。
采用多种方法研究了抗真菌活性，结果表明菊花和 Laggera pterodonta 的 EOs 对不同真菌的菌丝生长具有活性，但活性程度不同。棋盘试验表明，EOs 与 hymexazol 联合对腐霉有协同作用，对大部分目标病原菌表现出相加作用。此外，我们发现通过菌丝生长速率法，尖孢镰刀菌对 Laggera pterodonta EOs 的基础敏感性高于菊花。最后，体内模型证实了这些 EOs 作为植物农药的实用性，表明 EOs 可显著抑制由尖孢镰刀菌引起的三七根腐病的发生。
这些研究表明，菊花和 Laggera pterodonta 的 EOs 有可能开发成为保护珍贵中药材的新型无污染农药。
本研究为克服三七频发疾病提供了一种新的生物防治方法。
