School of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, 650500, P. R. China.
Lett Appl Microbiol. 2022 Jul;75(1):89-102. doi: 10.1111/lam.13706. Epub 2022 Apr 6.
To screen natural drugs with strong inhibitory effects against pathogenic fungi related to P. notoginseng, the antifungal activities of garlic and fennel EOs were studied by targeting P. notoginseng disease-associated fungi, and the possible action mechanisms of garlic and fennel EOs as plant fungicides were preliminarily discussed. At present, the antifungal mechanism of EOs has not been fully established. Therefore, understanding the antifungal mechanism of plant EOs is helpful to address P. notoginseng diseases continuous cropping disease-related obstacles and other agricultural cultivation problems. First, the Oxford cup method and chessboard were used to confirm that the EOs and oxamyl had a significant inhibitory effect on the growth of Fusarium oxysporum. F. oxysporum is the main pathogen causing root rot of P. notoginseng and the preliminary study on the antifungal mechanisms of the EOs against F. oxysporum showed that the inhibition of EOs mainly affects cell membrane permeability and cell processes and affects the enzyme activities of micro-organism, to achieve antifungal effects. Finally, an in vivo model verified that both two EOs could significantly inhibit the occurrence of root rot caused by F. oxysporum.
为了筛选对与三七相关病原真菌具有较强抑制作用的天然药物,本研究以三七病害相关真菌为靶标,研究了大蒜和茴香精油的抗真菌活性,并初步探讨了大蒜和茴香精油作为植物性杀菌剂的可能作用机制。目前,精油的抗真菌机制尚未完全建立。因此,了解植物精油的抗真菌机制有助于解决三七连作障碍等农业种植问题。首先,采用牛津杯法和棋盘法证实,精油和辛硫磷对尖孢镰刀菌的生长有显著抑制作用。尖孢镰刀菌是引起三七根腐病的主要病原菌,对精油对尖孢镰刀菌的抑菌机制的初步研究表明,精油的抑制作用主要影响细胞膜通透性和细胞过程,并影响微生物的酶活性,从而达到抑菌效果。最后,体内模型验证了两种精油均能显著抑制尖孢镰刀菌引起的根腐病的发生。