制备T淋巴细胞和B淋巴细胞受体特异性抗血清的方法。

Methods for producing T- and B-lymphocyte receptor-specific antisera.

作者信息

Krakowka S, Olsen R G, Cockerell G L

出版信息

J Immunol Methods. 1977;14(3-4):257-65. doi: 10.1016/0022-1759(77)90136-3.

PMID:320269

Abstract

Spent tissue culture medium from two continuous lymphoblastoid cell lines, FL-74 and CT45-S, expressing the T-lymphocyte receptor for guinea pig E and the B-lymphocyte receptor for EAC respectively were used to produce receptor-specific antisera. Anti-E receptor sera blocked E rosette formation on FL-74 cells, canine and feline lymphocytes and canine and feline thymocytes but not EAC rosette formation by CT45-S cells or canine and feline lymphocytes. Anti-EAC receptor sera blocked EAC rosette formation on CT45-S cells and canine or feline lymphocytes. Absorption of antisera will the appropriate lymphoblastoid cell line removed E or EAC-blocking activity. The results of this study suggest that similar methods may be used to produce lymphocyte subpopulation-specific antisera in other species including man.

摘要

分别表达豚鼠E的T淋巴细胞受体和EAC的B淋巴细胞受体的两种连续淋巴母细胞系FL-74和CT45-S的用过的组织培养基，被用于制备受体特异性抗血清。抗E受体血清阻断了FL-74细胞、犬和猫淋巴细胞以及犬和猫胸腺细胞上的E花环形成，但不阻断CT45-S细胞或犬和猫淋巴细胞上的EAC花环形成。抗EAC受体血清阻断了CT45-S细胞以及犬或猫淋巴细胞上的EAC花环形成。用相应的淋巴母细胞系吸收抗血清可去除E或EAC阻断活性。本研究结果提示，类似方法可用于在包括人类在内的其他物种中制备淋巴细胞亚群特异性抗血清。