在兔体内诱导特异性抗豚鼠T细胞血清

Induction of specific anti-guinea pig T cell sera in rabbits.

作者信息

Godfrey H P, Geczy A F, Gell P G, Rubin B

出版信息

J Immunol Methods. 1976;9(3-4):211-23. doi: 10.1016/0022-1759(76)90196-4.

Abstract

In an attempt to increase the specificity of antisera raised in rabbits against strain 2 guinea pig thymocytes and brain, the rabbits were screened for titres of natural antibodies to thymocytes and other lymphocytes. Although unimmunized rabbits commonly had moderate titres of cytotoxic antibodies to guinea pig thymocytes, occasional animals had low titres to thymocytes and moderate titres to bone marrow cells. Intravenous immunization of this latter group of rabbits with thymocytes led to the production of high titred anti-thymocyte sera which were easily made specific for thymus-derived lymphocytes (T cells) by absorption with L2 C lymphoma, a bone marrow-derived lymphoma of strain 2 guinea pigs. Sera raised against guinea pig brain in complete Freund's adjuvant which had high titres of antibodies to both thymocytes and bone marrow cells could be made specific for T cells only with great difficulty. The cytotoxic activity of the anti-T cell serum could be absorbed by strain 2 thymocytes and brain homogenates, while high dilutions of this serum inhibited the formation of spontaneous rosettes between guinea pig lymphoid cells and normal rabbit erythrocytes.

摘要

为了提高在兔体内产生的针对2号豚鼠胸腺细胞和脑的抗血清的特异性，对兔进行了天然抗体针对胸腺细胞和其他淋巴细胞的滴度筛选。尽管未免疫的兔通常对豚鼠胸腺细胞有中等滴度的细胞毒性抗体，但偶尔有动物对胸腺细胞滴度低而对骨髓细胞滴度中等。用胸腺细胞对后一组兔进行静脉免疫，产生了高滴度的抗胸腺细胞血清，通过用L2C淋巴瘤（2号豚鼠的一种骨髓源性淋巴瘤）吸收，很容易使其对胸腺来源的淋巴细胞（T细胞）具有特异性。在完全弗氏佐剂中产生的针对豚鼠脑的抗血清，对胸腺细胞和骨髓细胞都有高滴度抗体，很难使其仅对T细胞具有特异性。抗T细胞血清的细胞毒性活性可被2号胸腺细胞和脑匀浆吸收，而该血清的高稀释度可抑制豚鼠淋巴细胞与正常兔红细胞之间自发玫瑰花结的形成。