Becker Tobias, Kielkowski Pavel
Department Chemie, Ludwig-Maximilians-Universität München, Butenandtstraße 5-13, D-81377 München, Deutschland.
Biospektrum (Heidelb). 2020;26(7):743-746. doi: 10.1007/s12268-020-1491-2. Epub 2020 Nov 25.
Protein AMPylation is a prevalent protein post-translational modification in human cells involved in endoplasmic reticulum stress regulation and neural development. In this article we describe the design, synthesis and application of a pronucleotide probe suitable for fluorescence imaging and chemical protemics profiling of AMPylated proteins. Our probe utilizes straightforward strain-promoted azidealkyne click reaction for fluorescence labeling in living cells.
蛋白质腺苷酸化是人类细胞中一种普遍存在的蛋白质翻译后修饰，参与内质网应激调节和神经发育。在本文中，我们描述了一种适用于腺苷酸化蛋白质荧光成像和化学蛋白质组学分析的前体核苷酸探针的设计、合成及应用。我们的探针利用简单的应变促进叠氮-炔环加成反应在活细胞中进行荧光标记。
