[无可用内容]。
[Not Available].
作者信息
Becker Tobias, Kielkowski Pavel
机构信息
Department Chemie, Ludwig-Maximilians-Universität München, Butenandtstraße 5-13, D-81377 München, Deutschland.
出版信息
Biospektrum (Heidelb). 2020;26(7):743-746. doi: 10.1007/s12268-020-1491-2. Epub 2020 Nov 25.
Abstract
Protein AMPylation is a prevalent protein post-translational modification in human cells involved in endoplasmic reticulum stress regulation and neural development. In this article we describe the design, synthesis and application of a pronucleotide probe suitable for fluorescence imaging and chemical protemics profiling of AMPylated proteins. Our probe utilizes straightforward strain-promoted azidealkyne click reaction for fluorescence labeling in living cells.
摘要
蛋白质腺苷酸化是人类细胞中一种普遍存在的蛋白质翻译后修饰，参与内质网应激调节和神经发育。在本文中，我们描述了一种适用于腺苷酸化蛋白质荧光成像和化学蛋白质组学分析的前体核苷酸探针的设计、合成及应用。我们的探针利用简单的应变促进叠氮-炔环加成反应在活细胞中进行荧光标记。
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[Not Available].[无可用内容]。
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本文引用的文献
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FICD activity and AMPylation remodelling modulate human neurogenesis.FICD 活性和 AMP 化修饰调节人类神经发生。
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Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne [3 + 2] cycloaddition.使用铜（I）催化的叠氮化物-炔烃[3 + 2]环加成反应在体内进行基于活性的蛋白质谱分析。
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