Merson M H, Sack R B, Kibriya A K, Al-Mahmood A, Adamed Q S, Huq I
J Clin Microbiol. 1979 Apr;9(4):493-7. doi: 10.1128/jcm.9.4.493-497.1979.
Diagnosis of enterotoxigenic Escherichia coli diarrhea was made in 109 adult males with an acute dehydrating cholera-like syndrome in Dacca, Bangladesh, by testing 10 colonies isolated from admission stool specimens for production of heat-labile and heat-stable toxins. Toxin testing of one colony yielded a diagnosis in 92% of the cases, testing of two colonies yielded a diagnosis in 95% of the cases, testing of a pool of 5 colonies yielded a diagnosis in 95% of the cases, and testing of a pool of 10 colonies yielded a diagnosis in 96% of the cases. From stool cultures obtained on subsequent days, toxin testing of individual colonies and pools revealed diminished efficacy of pooling with decreasing numbers of enterotoxin-positive isolates in the pool. To detect the presence of enterotoxigenic E. coli in stools, toxin testing of 5 individual isolates and a pool of 10 colonies was found to be almost as effective as the testing of 10 individual isolates.
在孟加拉国达卡，对109名患有急性脱水霍乱样综合征的成年男性进行了产毒性大肠杆菌腹泻的诊断，方法是检测从入院粪便标本中分离出的10个菌落是否产生不耐热和耐热毒素。对一个菌落进行毒素检测，92%的病例可得出诊断结果；对两个菌落进行检测，95%的病例可得出诊断结果；对5个菌落的混合样本进行检测，95%的病例可得出诊断结果；对10个菌落的混合样本进行检测，96%的病例可得出诊断结果。在随后几天获得的粪便培养物中，对单个菌落和混合样本进行毒素检测发现，随着混合样本中肠毒素阳性分离株数量的减少，混合检测的效果也会降低。为了检测粪便中产毒性大肠杆菌的存在，发现对5个单个分离株和一个由10个菌落组成的混合样本进行毒素检测，其效果几乎与对10个单个分离株进行检测相同。
