Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 841456-83111, Iran; Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Science, Isfahan University of Medical Sciences, Isfahan 81745-359, Iran.
Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 841456-83111, Iran.
Int J Biol Macromol. 2020 May 15;151:220-229. doi: 10.1016/j.ijbiomac.2020.02.002. Epub 2020 Feb 3.
Hydrogel scaffolds have been frequently utilized due to their ability to absorb water and develop similar body cell conditions. Specific drug delivery to the tissues ensures less adverse side effects and more efficiency. In the present study, carboxymethyl chitosan (CMC)-methylcellulose (MC)-pluronic (P) and zinc chloride hydrogels containing meloxicam loaded into nanoparticles were developed and characterized. Nanoparticles were incorporated at 3.5, 4.5 and 5.5% (w/v). Hydrogels containing the same amounts of the meloxicam solution were also prepared. Gelation time, swelling and degradation of the hydrogels were investigated. Hydrogels were characterized by scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and rheological analysis. Meloxicam release, chondrocytes attachment and growth on the hydrogels were also studied. Gelation time, swelling and the degradation rate of the hydrogels were found to be decreased by nanoparticles and increased with the addition of the meloxicam solution. SEM images also showed three-dimensional networks. The ATR-FTIR bands were shifted to the lower wave numbers in the hydrogels containing nanoparticles and shifted to the upper ones in the hydrogels containing meloxicam solution. Storage (G') and loss (G″) modulus were increased by the nanoparticles and reduced by the meloxicam solution. 100% of meloxicam was released from the hydrogels containing the meloxicam solution within 20 days, but it was released slowly from the hydrogels containing nanoparticles in 37days. Chondrocytes metabolic activity was increased on the 6th and 10th days for all hydrogels. Hydrogel containing nanoparticles showed good biocompatibility, bioadhesion, cell growth and expansion. The hydrogel could be, therefore, suitable as a new composite biomaterial for the regeneration of articular cartilage and meloxicam delivery to control the pain and inflammation in osteoarthritis.
水凝胶支架因其能够吸收水分并模拟体内细胞环境而被广泛应用。将特定药物递送到组织中可以减少不良反应，提高效率。本研究制备并表征了载有负载美洛昔康的纳米粒子的羧甲基壳聚糖（CMC）-甲基纤维素（MC）-泊洛沙姆（P）和氯化锌水凝胶。将纳米粒子以 3.5%、4.5%和 5.5%（w/v）的比例掺入水凝胶中。同时，还制备了含有相同浓度美洛昔康溶液的水凝胶。考察了水凝胶的凝胶时间、溶胀和降解。通过扫描电子显微镜（SEM）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）和流变分析对水凝胶进行了表征。还研究了美洛昔康的释放、软骨细胞在水凝胶上的附着和生长。结果发现，纳米粒子的加入降低了水凝胶的凝胶时间、溶胀度和降解率，而美洛昔康溶液的加入则增加了这些参数。SEM 图像也显示了三维网络。ATR-FTIR 谱带在含有纳米粒子的水凝胶中向低波数移动，在含有美洛昔康溶液的水凝胶中向高波数移动。纳米粒子的加入增加了储存模量（G'）和损耗模量（G″），而美洛昔康溶液的加入则降低了这些模量。在含有美洛昔康溶液的水凝胶中，20 天内 100%的美洛昔康被释放，但在含有纳米粒子的水凝胶中，37 天内缓慢释放。所有水凝胶在第 6 天和第 10 天都增加了软骨细胞的代谢活性。含有纳米粒子的水凝胶表现出良好的生物相容性、生物黏附性、细胞生长和扩张。因此，该水凝胶可作为一种新的复合生物材料，用于关节软骨再生和美洛昔康递送，以控制骨关节炎的疼痛和炎症。
