Pre-Treatment and Finishing of Cellulosic Fabric Department, Textile Research Division, National Research Center, 33 El- Behooth St, Dokki, Giza 12311, Egypt.
Agricultural Botany Department, Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria 21531, Egypt.
Int J Biol Macromol. 2020 Apr 15;149:1304-1317. doi: 10.1016/j.ijbiomac.2020.02.004. Epub 2020 Feb 3.
Onion (Allium cepa L.) which is belonging to the family Liliaceae, is one of the greatest vital crops field worldwide. In this current work, Stored, high throughput and green synthesized silver nanoparticles; AgNPs (2000 ppm) were used as an eco-friendly nano fertilizer for onion in field conditions. All state of art analysis (Uv-vis, TEM, SEM, particle size analyzer and zeta potential) were used to characterize the formed AgNPs. Twelve concentrations (from 5 to 100 ppm) of AgNPs, were applied to onion, in addition to, two commercial nano products as control; iron nanoparticles (FeNPs) and zinc nanoparticles (ZnNPs) (2000 and 2500 ppm, as recommended by the markets). All concentrations were prepared under optical conditions and were applied three times in open field in a randomized complete block design (RCBD) during both seasons 2017/2018 and 2018/2019 as foliar application for onion plants after 25, 40 and 55 days, directly after the first irrigation of the plant. To this end, the physio-biochemical parameters such as CO concentration, stomatal conductance and internal CO were calculated via steady-state porometer 11 (LICOR, LI-1600, Lincoln, NE, USA). After 120 days of growth sowing (DAS), yield components and quality were evaluated. Just after harvesting, onion was subjected to extraction using dichloromethane for further analysis. All extracts were analyzed using GC-MS under the optimum operational condition to calculate the different constituents. The obtained results designated that, foliar application using 20 ppm of AgNPs displayed the highest mean values of all morphological, yield, yield components and quality characters compared with other concentrations and also to the commercial products, that used very high doses ranged from 2000 to 2500 ppm that can lead to genotoxicity in term of mutations in future, for human health. In conclusion, AgNPs can be used as an eco-friendly nano fertilizer with a recommended dose of 20 ppm which is considered a safe dose to the environment and human health too, compared with the crazy doses of the available commercial products.
洋葱（Allium cepa L.）属于百合科，是世界上最重要的蔬菜作物之一。在本研究中，使用经过储存、高通量和绿色合成的银纳米颗粒（AgNPs，2000 ppm）作为田间条件下洋葱的环保型纳米肥料。所有的现代分析技术（Uv-vis、TEM、SEM、粒度分析仪和zeta 电位）都用于对形成的 AgNPs 进行特征描述。十二种浓度（从 5 到 100 ppm）的 AgNPs 被应用于洋葱，此外，还有两种商业纳米产品作为对照；铁纳米颗粒（FeNPs）和锌纳米颗粒（ZnNPs）（市场推荐浓度为 2000 和 2500 ppm）。所有浓度均在光学条件下制备，并在 2017/2018 年和 2018/2019 年两个季节的开放田间条件下，根据随机完全区组设计（RCBD）进行三次叶面喷施，在植物第一次浇水后 25、40 和 55 天直接喷施洋葱植株。为此，通过稳态叶室 11（LICOR，LI-1600，Lincoln，NE，USA）计算 CO2 浓度、气孔导度和内部 CO2 等生理生化参数。在播种后 120 天（DAS），对产量构成和质量进行评估。收获后，立即用二氯甲烷提取洋葱，以备进一步分析。所有提取物均在最佳操作条件下通过 GC-MS 进行分析，以计算不同的成分。结果表明，与其他浓度和商业产品相比，20 ppm 的 AgNPs 叶面喷施表现出最高的所有形态、产量、产量构成和质量性状的平均值，而商业产品使用的剂量高达 2000 至 2500 ppm，可能会导致未来的基因突变，对人类健康造成遗传毒性。总之，AgNPs 可以作为一种环保型纳米肥料，推荐剂量为 20 ppm，与现有的商业产品的疯狂剂量相比，对环境和人类健康也被认为是安全的。
