Laboratory of Nutrition and Metabolic Regulation, Human Nutrition Unit, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, 7830490, Chile.
Nutrition Department, Faculty of Medicine, University of Chile, Santiago, 8380000, Chile.
Prostaglandins Leukot Essent Fatty Acids. 2020 Mar;154:102057. doi: 10.1016/j.plefa.2020.102057. Epub 2020 Jan 24.
Micronutrients (folates and vitamin B12) and long chain polyunsaturated fatty acids (LC-PUFAs) are linked through the one carbon cycle. We studied the effects of pre and postnatal high FA/low B12 diets (HFLB12) on hepatic fatty acid metabolism. Pregnant C57BL/6 mice were divided in two groups: control (2 mg folic acid: FA/25 µg vitamin B12/Kg food) and HFLB12 diets (8 mg FA/5 µg vitamin B12/Kg food). Offspring continued on the same diets until 60 days old. We determined hepatic fatty acid profile in dams and offspring and the expression of PPARα, Cpt-1, Acox-1 and Fas and the enzymatic activity of desaturases, all involved in lipid metabolism. In liver of dams, the HFHB12 diet decreased total fatty acids and desaturase activities; in offspring, effects were opposite, being more noticeable in females. Prenatal and postnatal unbalanced folic acid/B12 diets play a crucial role in regulating genes and enzymes involved in lipid metabolism in liver of dams and their offspring in adulthood.
微量营养素（叶酸和维生素 B12）和长链多不饱和脂肪酸（LC-PUFAs）通过一碳循环联系在一起。我们研究了产前和产后高 FA/低 B12 饮食（HFLB12）对肝脂肪酸代谢的影响。将怀孕的 C57BL/6 小鼠分为两组：对照组（2mg 叶酸：FA/25µg 维生素 B12/公斤食物）和 HFLB12 饮食组（8mg FA/5µg 维生素 B12/公斤食物）。后代继续食用相同的饮食，直到 60 天大。我们确定了母鼠和后代的肝脂肪酸谱以及 PPARα、Cpt-1、Acox-1 和 Fas 的表达，以及所有参与脂质代谢的去饱和酶的酶活性。在母鼠的肝脏中，HFHB12 饮食降低了总脂肪酸和去饱和酶的活性；在后代中，情况正好相反，雌性更为明显。产前和产后不平衡的叶酸/B12 饮食在调节母鼠及其成年后代肝脏中参与脂质代谢的基因和酶方面起着至关重要的作用。
