Kiselak Thomas D, Koerber Rachel, Verbeck Guido F
Department of Chemistry, The University of North Texas, Denton, TX, United States.
Forensic Sci Int. 2020 Mar;308:110173. doi: 10.1016/j.forsciint.2020.110173. Epub 2020 Jan 30.
This study focuses on the chemical route sourcing of illicitly produced Δ9-Tetrahydrocannabinol (Δ9-THC) via the acid-catalyzed cannabidiol isomerization reaction. Each of the acid-catalyzed reactions used acids that are readily available for the general population such as battery acid, muriatic acid, and vinegar. After the acid-catalyzed isomerization was complete, an analysis using Liquid Chromatography-coupled-Mass Spectrometry (LC-MS)-coupled-ion mobility to confirm all synthetic impurities in the sample was conducted. The conducted chemical route sourcing allows law enforcement to be able to determine how CBD was converted to psychoactive cannabinoids. Specifically, 10-methoxy-THC, 11-hydroxy-THC, 11,5″-dihydroxy-Δ9-THC, and 5″-hydroxy-CBD were able to be used as indicators in the determination of the chemical route sourcing. Additionally, the ion mobility allowed for a rapid secondary separation of the psychoactive cannabinoids without the need for the long LC/MS analysis time.
本研究聚焦于通过酸催化大麻二酚异构化反应非法生产Δ9-四氢大麻酚（Δ9-THC）的化学途径溯源。每个酸催化反应所使用的酸普通大众都很容易获取，如电瓶酸、盐酸和醋。在酸催化异构化完成后，采用液相色谱-质谱联用（LC-MS）-离子淌度对样品中的所有合成杂质进行分析以进行确认。所开展的化学途径溯源能让执法部门确定大麻二酚是如何转化为具有精神活性的大麻素的。具体而言，10-甲氧基-THC、11-羟基-THC、11,5″-二羟基-Δ9-THC和5″-羟基-CBD能够用作化学途径溯源测定中的指标。此外，离子淌度可对具有精神活性的大麻素进行快速二次分离，而无需长时间的LC/MS分析。
