Xie Zhangzhang, Lin Weitie, Luo Jianfei
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Science, Guangdong Academy of Science, Guangzhou 510650, PR China.
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China.
Bioresour Technol. 2021 Jun;330:124953. doi: 10.1016/j.biortech.2021.124953. Epub 2021 Mar 11.
To enhance microalgal lipid production, canonical two-step cultivation strategy that by transferring the microalgal cells grown in nutrient-replete medium to nutrient-depleted medium is widely used. However, the harvesting step during the transfer raises the production cost. To avoid the harvesting step, this study developed a continuous two-step (CTS) cultivation strategy. In the strategy, Chlorella sacchrarophila was grown in bioreactor while a xylanolytic bacterium Cellvibrio pealriver grown in an inner bag that embedded in the bioreactor; after the first-step co-cultivation, the inner bag is removed which then start the second-step cultivation of C. sacchrarophila. Based on the strategy, the lipid production was determined as 825.34-929.79 mg·L, which were 1.7-1.9 times higher than that of cultivation in canonical two-step strategy using glucose as feedstock. During the CTS strategy, the co-cultivation using xylan as feedstock promotes the microalgal growth and the removal of inner bag produces nutrient-depleted condition for enhancing microalgal lipid production.
为提高微藻脂质产量，一种典型的两步培养策略被广泛应用，即把在营养丰富培养基中生长的微藻细胞转移到营养耗尽的培养基中。然而，转移过程中的收获步骤增加了生产成本。为避免收获步骤，本研究开发了一种连续两步（CTS）培养策略。在该策略中，嗜糖小球藻在生物反应器中生长，而解木聚糖细菌珠江纤维弧菌在嵌入生物反应器的内袋中生长；第一步共培养后，取出内袋，然后开始嗜糖小球藻的第二步培养。基于该策略，脂质产量测定为825.34 - 929.79毫克·升，比以葡萄糖为原料的典型两步策略培养高出1.7 - 1.9倍。在CTS策略中，以木聚糖为原料的共培养促进了微藻生长，取出内袋产生了营养耗尽的条件以提高微藻脂质产量。
