Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, A. Abrahama 58, 80-307 Gdańsk, Poland.
Sensors (Basel). 2020 Feb 3;20(3):813. doi: 10.3390/s20030813.
Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user's safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.
由于诺如病毒是全世界急性胃肠炎的主要病因，因此在医学诊断中快速检测它至关重要。在这项工作中，报道了一种用于检测诺如病毒病毒样颗粒（VLPs）的快速、灵敏和选择性光纤生物传感器。该传感器基于涂覆有针对诺如病毒主要外壳蛋白的抗体的高灵敏度长周期光纤光栅（LPFG）。验证了几种修饰方法，以获得将蛋白质受体可靠地固定在 LPFG 表面上的方法。我们能够以无标记的方式在 40 分钟的测定中检测到 1ng/mL 的诺如病毒 VLPs。由于应用光纤作为传感器，有可能通过将测量点与信号处理装置分离来提高使用者的安全性。此外，我们的传感器体积小、重量轻，并且所提出的测定方法简单直接。设计的基于 LPFG 的生物传感器可用于快速检测诺如病毒和疫苗测试。
