Ultrafast Detection of Peroxynitrite in Parkinson's Disease Models Using a Near-Infrared Fluorescent Probe.

Parkinson's disease (PD) is a neurodegenerative disease that devastatingly affects people's lives. Numerous research studies have shown that peroxynitrite (ONOO) plays a pivotal role in the pathogenesis of PD. However, a suitable tool that could quickly and sensitively detect ONOO in various PD models is still lacking. To this end, we designed and synthesized a series of near-infrared probes that could detect ONOO within seconds by near-infrared fluorescent imaging in an ultrafast and highly selective manner. It is noteworthy that one of those developed probes, , showed excellent sensing performance and blood-brain barrier penetrating ability. was successfully applied for imaging of ONOO fluxes in multiple PD models including PC12 cell, Drosophila, , and mouse brain, indicating its great potential application not only for understanding the biological roles that ONOO played in PD but also for early PD diagnosis and treatment.