Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, P. R. China.
College of Materials Science and Engineering, Nanjing Forestry University, 159 Longpan Road, Xuanwu District, Nanjing 210037, P. R. China.
Anal Chem. 2020 Mar 3;92(5):4038-4045. doi: 10.1021/acs.analchem.9b05599. Epub 2020 Feb 20.
Parkinson's disease (PD) is a neurodegenerative disease that devastatingly affects people's lives. Numerous research studies have shown that peroxynitrite (ONOO) plays a pivotal role in the pathogenesis of PD. However, a suitable tool that could quickly and sensitively detect ONOO in various PD models is still lacking. To this end, we designed and synthesized a series of near-infrared probes that could detect ONOO within seconds by near-infrared fluorescent imaging in an ultrafast and highly selective manner. It is noteworthy that one of those developed probes, , showed excellent sensing performance and blood-brain barrier penetrating ability. was successfully applied for imaging of ONOO fluxes in multiple PD models including PC12 cell, Drosophila, , and mouse brain, indicating its great potential application not only for understanding the biological roles that ONOO played in PD but also for early PD diagnosis and treatment.
帕金森病（PD）是一种神经退行性疾病，严重影响人们的生活。大量研究表明，过氧亚硝酸盐（ONOO）在 PD 的发病机制中起着关键作用。然而，目前仍然缺乏一种能够快速、灵敏地检测各种 PD 模型中 ONOO 的合适工具。为此，我们设计并合成了一系列近红外探针，能够通过近红外荧光成像在几秒钟内快速、高选择性地检测 ONOO。值得注意的是，其中一种开发的探针 ，表现出优异的传感性能和血脑屏障穿透能力。 成功应用于包括 PC12 细胞、果蝇、 和小鼠大脑在内的多种 PD 模型中的 ONOO 通量成像，表明其不仅具有用于理解 ONOO 在 PD 中发挥的生物学作用的巨大潜在应用价值，而且具有早期 PD 诊断和治疗的巨大潜力。
