Gotoh E, Yamagami T, Yamamoto H, Okamoto H
Department of Biochemistry, Tohoku University School of Medicine, Sendai, Japan.
Biochem Int. 1988 Sep;17(3):555-62.
We determined the chromosomal location of the human VIP/PHM-27 gene in two different ways. First, we performed chromosome sorting with a cell sorter. Sufficient numbers of chromosomes for sorting were obtained by cultivating peripheral blood lymphocytes with interleukin-2. Spot blot hybridization of a molecularly cloned fragment of the gene to the sorted chromosomes revealed that this gene is located on chromosome 6. To define the location more precisely, in situ hybridization of the same fragment to the metaphase spreads was then performed, and human VIP/PHM-27 gene was assigned to a region near the terminus of the long arm of chromosome 6 (q26----q27).
我们用两种不同的方法确定了人类血管活性肠肽/PHM - 27基因的染色体定位。首先，我们使用细胞分选仪进行染色体分选。通过用白细胞介素-2培养外周血淋巴细胞获得了足够数量用于分选的染色体。将该基因的分子克隆片段与分选后的染色体进行斑点杂交，结果显示该基因位于6号染色体上。为了更精确地确定其位置，随后将同一片段与中期染色体铺展进行原位杂交，结果将人类血管活性肠肽/PHM - 27基因定位于6号染色体长臂末端附近的一个区域（q26----q27）。
