College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, 430072, Hubei Province, China.
Zhongnan Hospital, Wuhan University, Wuhan, China.
Anal Chim Acta. 2020 Mar 8;1101:169-175. doi: 10.1016/j.aca.2019.12.027. Epub 2019 Dec 26.
Circular RNA is a novel class of endogenous non-coding RNA that has a well-conserved sequence. Emerging evidence has shown that circRNA is implicated in various biological processes, therefore, the ability to quantify circRNA in real samples facilitates the elucidation of circRNA related regulatory circuits and its disease associations. Here, a reverse transcription mediated rolling circle amplification process was proposed to achieve selective amplification of the target circRNA. This assay, termed reverse transcription-rolling circle amplification (RT-RCA), can be used to identify circRNA with high selectivity within only a few hours. RT-RCA is a competitive candidate technique for circRNA detection and may help us to understand the role of circRNA in cellular processes and human diseases in more detail.
环状 RNA 是一类新型的内源性非编码 RNA，具有高度保守的序列。越来越多的证据表明，环状 RNA 参与了多种生物学过程，因此，能够在实际样本中定量检测环状 RNA 有助于阐明环状 RNA 相关的调控回路及其与疾病的关联。在这里，我们提出了一种通过逆转录介导的滚环扩增过程来实现靶标环状 RNA 选择性扩增的方法。这种方法被称为逆转录-滚环扩增（RT-RCA），仅需数小时即可实现对环状 RNA 的高选择性识别。RT-RCA 是一种环状 RNA 检测的竞争性候选技术，可能有助于我们更详细地了解环状 RNA 在细胞过程和人类疾病中的作用。
