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本文引用的文献

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Systems structural biology measurements by in vivo cross-linking with mass spectrometry.通过体内交联和质谱法进行系统结构生物学测量。
Nat Protoc. 2019 Aug;14(8):2318-2343. doi: 10.1038/s41596-019-0181-3. Epub 2019 Jul 3.
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Chemical cross-linking with mass spectrometry: a tool for systems structural biology.化学交联与质谱联用：系统结构生物学的研究工具。
Curr Opin Chem Biol. 2019 Feb;48:8-18. doi: 10.1016/j.cbpa.2018.08.006. Epub 2018 Aug 30.
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Acinetobacter baumannii OmpA Is a Selective Antibiotic Permeant Porin.鲍曼不动杆菌外膜蛋白A是一种选择性抗生素通透孔蛋白。
ACS Infect Dis. 2018 Mar 9;4(3):373-381. doi: 10.1021/acsinfecdis.7b00168. Epub 2017 Dec 26.
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Chemical Crosslinking Mass Spectrometry Analysis of Protein Conformations and Supercomplexes in Heart Tissue.心脏组织中蛋白质构象和超复合物的化学交联质谱分析。
Cell Syst. 2018 Jan 24;6(1):136-141.e5. doi: 10.1016/j.cels.2017.10.017. Epub 2017 Nov 29.
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Virulence Traits: A Comparative Study of a Novel Sequence Type with Other Italian Endemic International Clones.毒力特征：一种新型序列型与其他意大利地方性国际克隆株的比较研究。
Front Microbiol. 2017 Oct 12;8:1977. doi: 10.3389/fmicb.2017.01977. eCollection 2017.
6
Outer membrane protein A contributes to antimicrobial resistance of Acinetobacter baumannii through the OmpA-like domain.外膜蛋白 A 通过 OmpA 样结构域促进鲍曼不动杆菌的抗菌药物耐药性。
J Antimicrob Chemother. 2017 Nov 1;72(11):3012-3015. doi: 10.1093/jac/dkx257.
7
Large-Scale and Targeted Quantitative Cross-Linking MS Using Isotope-Labeled Protein Interaction Reporter (PIR) Cross-Linkers.使用同位素标记的蛋白质相互作用报告分子（PIR）交联剂进行大规模靶向定量交联质谱分析
J Proteome Res. 2017 Feb 3;16(2):720-727. doi: 10.1021/acs.jproteome.6b00752. Epub 2016 Nov 30.
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Mitochondrial protein interactome elucidated by chemical cross-linking mass spectrometry.通过化学交联质谱法阐明线粒体蛋白质相互作用组。
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1732-1737. doi: 10.1073/pnas.1617220114. Epub 2017 Jan 27.
9
In vivo protein interaction network analysis reveals porin-localized antibiotic inactivation in Acinetobacter baumannii strain AB5075.体内蛋白质相互作用网络分析揭示了鲍曼不动杆菌 AB5075 菌株中孔蛋白定位的抗生素失活。
Nat Commun. 2016 Nov 11;7:13414. doi: 10.1038/ncomms13414.
10
In Vivo Conformational Dynamics of Hsp90 and Its Interactors.热休克蛋白 90 及其相互作用物的体内构象动力学。
Cell Chem Biol. 2016 Jun 23;23(6):716-26. doi: 10.1016/j.chembiol.2016.05.012.

体内交联 MS 揭示了 OmpA 与不同类别的β-内酰胺酶之间的连接的保守性。

In Vivo Cross-Linking MS Reveals Conservation in OmpA Linkage to Different Classes of β-Lactamase Enzymes.

机构信息

Department of Genome Sciences University of Washington School of Medicine 850 Republican Street, Brotman Building, Room 154 Seattle , Washington 98109 , United States.

出版信息

J Am Soc Mass Spectrom. 2020 Feb 5;31(2):190-195. doi: 10.1021/jasms.9b00021. Epub 2019 Nov 21.

DOI:10.1021/jasms.9b00021
PMID:32031408
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7970438/
Abstract

Molecular interactions between two different classes of β-lactamase enzymes and outer membrane protein A (OmpA) were studied by in vivo chemical cross-linking of a multi-drug-resistant strain of AB5075. Class A β-lactamase blaGES-11 and Class D β-lactamase Oxa23, responsible for hydrolysis of different types of β-lactam antibiotics, were found to be cross-linked to similar lysine sites of the periplasmic domain of outer membrane protein OmpA, despite low sequence homology between the two enzymes. The findings from in vivo XL-MS suggest that the interacting surfaces between both β-lactamase enzymes and OmpA are conserved during molecular evolution, and the OmpA C-terminus domain serves an important function of anchoring different types of β-lactamase enzymes in the periplasmic space.

摘要

通过对多药耐药株 AB5075 的体内化学交联研究，研究了两种不同类别的β-内酰胺酶和外膜蛋白 A（OmpA）之间的分子相互作用。负责水解不同类型β-内酰胺抗生素的类 Aβ-内酰胺酶 blaGES-11 和类 Dβ-内酰胺酶 Oxa23 被发现与外膜蛋白 OmpA 的周质域的类似赖氨酸位点交联，尽管两种酶之间的序列同源性较低。体内 XL-MS 的研究结果表明，在分子进化过程中，两种β-内酰胺酶与 OmpA 之间的相互作用表面是保守的，OmpA C 末端结构域在周质空间中锚定不同类型的β-内酰胺酶方面发挥着重要作用。